Complete Freund's adjuvant (CFA)
Based on 22 publication(s) in Google Scholar
Complete Freund's adjuvant (CFA) is an immunoadjuvant emulsified with antigen by its discoverer Jules T. Freund to enhance an animal's immune response to an antigen. Complete Freund's adjuvant (CFA) is also an inducer of the Th1 immune response and a ligand of TLRs. Complete Freund's adjuvant (CFA) contains heat-killed inactive tuberculosis bacilli and consists of a paraffin oil-in-water emulsion. Complete Freund's adjuvant (CFA) stimulates a strong and durable immune response and can be used to induce persistent inflammatory pain models in mice, experimental autoimmune myocarditis (EAM) models, and more. Incomplete Freund's adjuvant (IFA) (HY-153808A) is another type of Freund's Adjuvant that stimulates a weaker immune response.
For research use only. We do not sell to patients.
- CAS No.: 9007-81-2
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Storage:
Store at 4°C, protect from light, do not freeze
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Complete Freund's adjuvant (CFA)
More- Adv Funct Mater. 2025 Mar 10.
- Nat Commun. 2026 May 19;17(1):4418. [Abstract]
- Acta Pharm Sin B. 2025 Oct 31.
- Int J Biol Sci. 2024 Sep 16;20(12):4957-4977. [Abstract]
- Phytomedicine. 2026 Aug:158:158406. [Abstract]
- Adv Healthc Mater. 2025 Jul 16:e2501440. [Abstract]
- Food Res Int. 2025 Dec;222(Pt 1):117620. [Abstract]
- J Funct Biomater. 2026 May 6;17(5):228. [Abstract]
- Int J Mol Sci. 2026 May 11;27(10):4270. [Abstract]
- Biomolecules. 2026 May;16(5):642. [Abstract]
- Int Immunopharmacol. 2024 Jul 12:139:112646. [Abstract]
- ACS Omega. 2026 Jan 21;11(4):5853-5864. [Abstract]
- J Neurochem. 2025 Sep;169(9):e70235. [Abstract]
- Front Biosci (Landmark Ed). 2025 May 23;30(5):37838. [Abstract]
- Viruses. 2026 May 21;18(5):579. [Abstract]
- Mol Pain. 2025 Jan-Dec:21:17448069251324645. [Abstract]
- Chem Biodivers. 2025 Feb;22(2):e202401539. [Abstract]
- J Cardiovasc Transl Res. 2025 Apr;18(2):237-246. [Abstract]
- Gen Comp Endocrinol. 2026 May 12;381:114947.
- Folia Histochem Cytobiol. 2025;63(1):11-27. [Abstract]
- Med Mol Morphol. 2025 Jun;58(2):114-125. [Abstract]
- SSRN. 2025 Oct 10.
Biological Activity
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Animal Model:A/J, BALB/c, and IL6KO BALB/c male mice[1]
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Dosage:
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Administration:Subcutaneous Injectionon (sc); injected at days 0 and 7. EAM severity was analyzed by histology on day 21 (Repeated administration of CFA to animals can cause multiple injection-site effects, the most often observed are on site granulomas and pain)
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Result:Increased the relative proportion of CD11b+F4/80−monocytes and CD11b+Gr1+ granulocytes.
Expanded monocytes in the heart and in the spleen and those monocytes in the spleen make less IL-10 as well as more IL-6.
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Complete Freund's adjuvant (CFA) induces experimental autoimmune myocarditis and neuropathic pain, with a relatively low plasma clearance rate (22%)[1][2][3][4].
Administration: 5mg/ml • s.c. • twice
(2) On days 0 and 7, mice received subcutaneous injections peptide emulsified in CFA supplemented with 5 mg/mL of Mycobacterium tuberculosis, strain H37Ra.
Administration: 20 μl • s.c. • once
(2) Tests PWT and PWL for both hind paws corresponding to before and 24, 48, 72 h after CFA injection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9007-81-2
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Complete Freund's adjuvant (CFA)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at 4°C, protect from light, do not freeze
Publications (22)
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Journal Impact Factor
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Most Recent
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Nat Commun
Novelty exploration-activated ensemble in the lateral hypothalamus confers analgesic and anxiolytic effects. [Abstract]2026 May 19;17(1):4418. PMID: 42156381 -
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Int J Biol Sci
NDR1/FBXO11 promotes phosphorylation-mediated ubiquitination of β-catenin to suppress metastasis in prostate cancer. [Abstract]2024 Sep 16;20(12):4957-4977. PMID: 39309441 -
Phytomedicine
Bioactive compound emodin from clinical formula Zhi-Lou-Xun-Xi decoction exerts rapid analgesic effects by targeting TRPV1. [Abstract]2026 Aug:158:158406. PMID: 42275875 -
Adv Healthc Mater
Triple-Tailored Analgesic Hydrogel System Targeting ADAM17 in Orofacial Inflammatory Pain. [Abstract]2025 Jul 16:e2501440. PMID: 40671384 -
Food Res Int
Glycyrrhiza uralensis Fisch: A novel source of analgesic activity through NaV1.8 sodium channel modulation. [Abstract]2025 Dec;222(Pt 1):117620. PMID: 41267240 -
J Funct Biomater
Therapeutic Efficacy of Dual-Targeting Nanoparticles with Low Immunogenicity in the Treatment of Rheumatoid Arthritis. [Abstract]2026 May 6;17(5):228. PMID: 42188395 -
Int J Mol Sci
Establishment of an Autoimmune Premature Ovarian Insufficiency Mouse Model with Proteomic Analyses: An Exploratory Study. [Abstract]2026 May 11;27(10):4270. PMID: 42196258 -
Biomolecules
AjTEAD1 Targets AjCyclin E to Promote Cell Proliferation During Intestinal Regeneration in Apostichopus japonicus. [Abstract]2026 May;16(5):642. PMID: 42193993 -
Int Immunopharmacol
A novel PROTAC molecule dBET1 alleviates pathogenesis of experimental autoimmune encephalomyelitis in mice by degrading BRD4. [Abstract]2024 Jul 12:139:112646. PMID: 39002520 -
ACS Omega
ROS-Scavenging Bamboo-Derived Carbon Dot-Methotrexate Nanocomposite Ameliorates Rheumatoid Arthritis through Dual Therapeutic Mechanisms. [Abstract]2026 Jan 21;11(4):5853-5864. PMID: 41658163 -
J Neurochem
Ospemifene, a Selective Estrogen Receptor Modulator, Enhances Oligodendrocyte Myelination and Preserves Neurofunctions Against Injuries. [Abstract]2025 Sep;169(9):e70235. PMID: 40959993 -
Front Biosci (Landmark Ed)
HMGB1 Mediates Microglia-Astrocyte/Neuron Crosstalk and Pyroptosis by the TLR4/NF-κB Pathway in Multiple Sclerosis. [Abstract]2025 May 23;30(5):37838. PMID: 40464519 -
Viruses
Aerosol Inhalation of a Recombinant H7N9 Hemagglutinin Antigen Elicits Systemic and Mucosal Immune Responses in Mice. [Abstract]2026 May 21;18(5):579. PMID: 42198780 -
Mol Pain
Upregulation of LRRC8A in the anterior cingulate cortex mediates chronic visceral pain in adult male mice with neonatal maternal deprivation. [Abstract]2025 Jan-Dec:21:17448069251324645. PMID: 39962353 -
Chem Biodivers
Cornus Officinalis Total Glycosides Alleviate Granulomatous Lobular Mastitis via the B7-CD28/CTLA-4 Costimulatory Pathway. [Abstract]2025 Feb;22(2):e202401539. PMID: 39344790 -
J Cardiovasc Transl Res
Drp1 Promotes Macrophage M1 Polarization and Inflammatory Response in Autoimmune Myocarditis by Driving Mitochondrial Fission. [Abstract]2025 Apr;18(2):237-246. PMID: 39388091 -
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Folia Histochem Cytobiol
Inhibition of STAT1 alleviates oxidative damage in retinal pigment epithelial cells and exhibits neuroprotective effects in autoimmune optic neuritis by upregulating IFI30 lysosomal thiol reductase. [Abstract]2025;63(1):11-27. PMID: 40152123 -
Med Mol Morphol
Investigation of the molecular mechanism of quercetin in inhibiting ankylosing spondylitis ossification via the bone morphogenetic protein/smad signaling pathway. [Abstract]2025 Jun;58(2):114-125. PMID: 39722109 -
Purity & Documentation
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Data Sheet (276 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Fontes JA, et al. Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL-6 production during initiation of the immune response. Immun Inflamm Dis. 2017 Jun;5(2):163-176. [Content Brief]
[2]. Ji YW, et al. Plasticity in ventral pallidal cholinergic neuron-derived circuits contributes to comorbid chronic pain-like and depression-like behaviour in male mice. Nat Commun. 2023 Apr 17;14(1):2182. [Content Brief]
[3]. Sun T, et al. Gastrodin relieved complete Freund's adjuvant-induced spontaneous pain by inhibiting inflammatory response. Int Immunopharmacol. 2016 Dec;41:66-73. [Content Brief]
[4]. Projean D, et al. Use of Freund's Complete Adjuvant (FCA) in inflammatory pain models: consequences on the metabolism and pharmacokinetics of the non-peptidic delta receptor agonist SNC80 in the rat. Xenobiotica. 2007 Aug;37(8):870-83. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Complete Freund's adjuvant (CFA) Related Classifications
HY-153808 Related Classifications
Powered by Bioz
- Complete Freund's adjuvant (CFA)
- 9007-81-2
- Toll-like Receptor (TLR)
- immunologic adjuvant
- immunity
- inflammation
- immunization-induced animal models
- collagen-induced arthritis
- CIA
- experimental autoimmune encephalomyelitis
- EAE
- experimental autoimmune thyroiditis
- EAT
- experimental autoimmune uveitis
- EAU
- experimental autoimmune myocarditis
- EAM
- Inhibitor
- inhibitor
- inhibit