2. Academic Validation
  3. 1,4- β-d-Glucomannan from Dendrobium officinale Activates NF- к B via TLR4 to Regulate the Immune Response

1,4- β-d-Glucomannan from Dendrobium officinale Activates NF- к B via TLR4 to Regulate the Immune Response

  • Molecules. 2018 Oct 16;23(10):2658. doi: 10.3390/molecules23102658.
Yan-Ping Huang 1 2 3,  Tao-Bin He 4 5 6 7,  Xian-Dan Cuan 8 9 10,  Xuan-Jun Wang 11 12 13,  Jiang-Miao Hu 14,  Jun Sheng 15 16 17
Affiliations

Affiliations

  • 1 Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 2 College of Science, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 3 College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 4 Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 5 College of Science, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 6 College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 7 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. [email protected].
  • 8 Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 9 College of Science, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 10 College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 11 Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 12 College of Science, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 13 College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 14 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. [email protected].
  • 15 Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 16 College of Science, Yunnan Agricultural University, Kunming 650201, China. [email protected].
  • 17 College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. [email protected].
PMID: 30332800 DOI: 10.3390/molecules23102658
Abstract

2,3-O-acetylated-1,4-β-d-glucomannan (DOP-1-1) is a polysaccharide isolated from the stem of Dendrobium officinale. DOP-1-1 has been demonstrated to have remarkable immunomodulatory properties, but little is known about the influence of its structural diversity on bioactivity (and even less about the exact mechanism underlying its immune responses). First, DOP-1-1 was stabilized at different temperatures and pH conditions based on differential scanning calorimetry and size exclusion-chromatography⁻high-performance liquid chromatography. Then, a detailed study on the effects of DOP-1-1 on a human leukemia monocytic cell line (THP-1) under normal conditions was undertaken. DOP-1-1 promoted the translocation of nuclear factor-kappa B (NF-κB) and degradation of IκB proteins. The expression of genes and proteins closely associated with the immune, survival and apoptotic functions of NF-κB were analyzed by quantitative real-time RT-PCR. Furthermore, CCL4 and IP10 were confirmed to be the novel targets of the immune response stimulated by DOP-1-1. The phosphorylation of NF-кB was inhibited by treatment with a Toll-like Receptor 4 (TLR4) antagonist (TAK-242) and myeloid differentiation factor 88 (MyD88) inhibitor (ST2825). These data suggested: (i) the O-acetylated glucomannan DOP-1-1 is present in the steady state in low-pH solutions; (ii) DOP-1-1 can induce an immune response through NF-кB mediated by a TLR4 signaling pathway; and (iii) CCL4 and IP10 could be the novel targets of the immune response stimulated by O-acetylated glucomannan.

Keywords

1,4-β-d-glucomannan; Dendrobium officinale; NF-кB; TLR4; thermal stability.

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