2. Academic Validation
  3. Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry

Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry

  • STAR Protoc. 2021 Sep 27;2(4):100848. doi: 10.1016/j.xpro.2021.100848.
Akiko Ogawa 1,  Fan-Yan Wei 1
Affiliations

Affiliation

  • 1 Department of Modomics Biology and Medicine, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai 980-8575, Japan.
PMID: 34622220 DOI: 10.1016/j.xpro.2021.100848
Abstract

About 150 modifications have been identified in RNA species. Besides their regulatory roles in the intracellular gene expression, abundant modified RNA nucleosides are catabolized from RNA and released into extracellular fluids, which can impact extracellular signaling as ligands for receptors. Here, we describe a protocol to prepare samples from biological specimens, including cultured cells, extracellular fluid, and tissues, to measure both intracellular and extracellular RNA modifications using mass spectrometry. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2021).

Keywords

Mass Spectrometry; Metabolism; Metabolomics; Molecular Biology; Signal Transduction.

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