2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. N2,N2-Dimethylguanosine

N2,N2-Dimethylguanosine is a methylated modified nucleoside present in RNA and serves as a structural modification component of tRNA. N2,N2-Dimethylguanosine inhibits reverse transcriptase-mediated cDNA synthesis and is one of the key modifications affecting sequencing efficiency in high-throughput RNA sequencing. N2,N2-Dimethylguanosine can be selectively demethylated at one methyl group by AlkB mutant enzymes (such as D135S/L118V) and converted to N2-methylguanosine, thereby reducing the inhibition of reverse transcription.

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N2,N2-Dimethylguanosine

N2,N2-Dimethylguanosine Chemical Structure

CAS No. : 2140-67-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Based on 4 publication(s) in Google Scholar

Other Forms of N2,N2-Dimethylguanosine:

N2,N2-Dimethylguanosine Related Antibodies

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Description

N2,N2-Dimethylguanosine is a methylated modified nucleoside present in RNA and serves as a structural modification component of tRNA. N2,N2-Dimethylguanosine inhibits reverse transcriptase-mediated cDNA synthesis and is one of the key modifications affecting sequencing efficiency in high-throughput RNA sequencing. N2,N2-Dimethylguanosine can be selectively demethylated at one methyl group by AlkB mutant enzymes (such as D135S/L118V) and converted to N2-methylguanosine, thereby reducing the inhibition of reverse transcription[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

N2,N2-dimethylguanosine (5-95 μM for 12m22GA; 2.3-84 μM for 13m22GA; 2.9-105 μM for 11m22GA) increases hairpin stability by 3.5°C in the 12m22GA oligoribonucleotide, slightly destabilizes hairpin stability by 1°C and significantly destabilizes duplex stability in the 13m22GA oligoribonucleotide, and shifts the secondary structure equilibrium toward a mixture of hairpin and duplex forms in the 11m22GA oligoribonucleotide[1].
N2,N2-dimethylguanosine (0.5 mM; ~1 week at 291 K) forms imino-hydrogen bonded pairs with A in the 13m22GA RNA duplex, inducing local conformational changes including helical bending and altered hydrogen bond geometry, and reducing minor groove hydration, which contributes to reduced duplex thermodynamic stability[1].
N2,N2-dimethylguanosine (m22G) (1 nM; 2 h) in a synthetic 9mer RNA oligo substrate is fully converted to N2-methylguanosine (m2G) via selective removal of one methyl group by the AlkBD135S/L118V mutant, with no further demethylation to unmodified guanosine[2].
N2,N2-dimethylguanosine at position 26 (m22G26) in HEK293T cell tRNAs has its modification index reduced via selective demethylation to N2-methylguanosine (m2G) by the AlkB D135S/L118V mutant (as part of a three-demethylase mixture), with the greatest effect observed in mitochondrial tRNAIle and cytosolic tRNAThr, tRNATrp, and tRNATyr[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N2,N2-Dimethylguanosine Related Antibodies
Molecular Weight

311.29

Formula

C12H17N5O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N(C=N2)C3=C2C(NC(N(C)C)=N3)=O)O1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 15 mg/mL (48.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2124 mL 16.0622 mL 32.1244 mL
5 mM 0.6425 mL 3.2124 mL 6.4249 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.5 mg/mL (4.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.5 mg/mL (4.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2124 mL 16.0622 mL 32.1244 mL 80.3110 mL
5 mM 0.6425 mL 3.2124 mL 6.4249 mL 16.0622 mL
10 mM 0.3212 mL 1.6062 mL 3.2124 mL 8.0311 mL
15 mM 0.2142 mL 1.0708 mL 2.1416 mL 5.3541 mL
20 mM 0.1606 mL 0.8031 mL 1.6062 mL 4.0155 mL
25 mM 0.1285 mL 0.6425 mL 1.2850 mL 3.2124 mL
30 mM 0.1071 mL 0.5354 mL 1.0708 mL 2.6770 mL
40 mM 0.0803 mL 0.4016 mL 0.8031 mL 2.0078 mL
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N2,N2-Dimethylguanosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N2,N2-Dimethylguanosine2140-67-2Endogenous Metabolitemitochondrial tRNAIleAlkB D135S/L118V mutantRNA sequencingHEK293T celleukaryotic tRNAsN2-methylguanosinetRNAcDNA synthesisRNA duplexesnucleoside modificationInhibitorinhibitorinhibit

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