Cat. No.: HY-113139

1-Methylinosine

(Synonyms: N1-Methylinosine)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

1-Methylinosine (N1-MetHYlinosine) is a modified nucleotide located at position 37 of eukaryotic tRNA, 3' to the tRNA anticodon. 1-Methylinosine is a minor metabolite of 1-methyladenosine (HY-113081). The level of 1-Methylinosine is significantly elevated in urine samples from breast cancer models.

For research use only. We do not sell to patients.

Purity: 99.72%
CAS No.: 2140-73-0
Formula: C₁₁H₁₄N₄O₅
Molecular Weight:282.25

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

1-Methylinosine Chemical Structure

CAS No.: 2140-73-0

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Biological Activity
- Description
- IC₅₀ & Target
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vivo

1-Methylinosine is a minor endogenous metabolite of 1-methyladenosine in male Sprague-Dawley rats, accounting for approximately 2.6% of the total excreted radioactivity following intravenous administration of 1-methyladenosine^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2140-73-0
Appearance Solid
Molecular Weight 282.25
Formula C₁₁H₁₄N₄O₅
Color White to off-white
SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=CN(C)C3=O)=C3N=C2)O1
Synonyms

N1-Methylinosine
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

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Journal Impact Factor

Cell Chem Biol

MapID-based quantitative mapping of chemical modifications and expression of human transfer RNA. [Abstract]

2025 May 15;32(5):752-766.e7. PMID: 40318625
Talanta

High-throughput profiling of RNA modifications by ultra-performance liquid chromatography coupled to complementary mass spectrometry: Methods, quality control, and applications. [Abstract]

2023 Oct 1:263:124697. PMID: 37262985
bioRxiv

2023 Feb 2.

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (295.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5430 mL

17.7148 mL

35.4296 mL

5 mM

0.7086 mL

3.5430 mL

7.0859 mL

10 mM

0.3543 mL

1.7715 mL

3.5430 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tormey DC, et al. Biological markers in breast carcinoma—clinical correlations with pseudouridine, n2, N2‐dimethylguanosine, and 1‐methylinosine. Journal of surgical oncology. 1980 Jul;14(3):267-73.

[2]. Dutta SP, Chheda GB. Metabolism of 1-methyladenosine. Biochem Med Metab Biol. 1987 Aug;38(1):69-73.
[Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5430 mL	17.7148 mL	35.4296 mL	88.5740 mL
	5 mM	0.7086 mL	3.5430 mL	7.0859 mL	17.7148 mL
	10 mM	0.3543 mL	1.7715 mL	3.5430 mL	8.8574 mL
	15 mM	0.2362 mL	1.1810 mL	2.3620 mL	5.9049 mL
	20 mM	0.1771 mL	0.8857 mL	1.7715 mL	4.4287 mL
	25 mM	0.1417 mL	0.7086 mL	1.4172 mL	3.5430 mL
	30 mM	0.1181 mL	0.5905 mL	1.1810 mL	2.9525 mL
	40 mM	0.0886 mL	0.4429 mL	0.8857 mL	2.2143 mL
	50 mM	0.0709 mL	0.3543 mL	0.7086 mL	1.7715 mL
	60 mM	0.0590 mL	0.2952 mL	0.5905 mL	1.4762 mL
	80 mM	0.0443 mL	0.2214 mL	0.4429 mL	1.1072 mL
	100 mM	0.0354 mL	0.1771 mL	0.3543 mL	0.8857 mL

Keywords

1-Methylinosine

3 Cited Publications

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1-Methylinosine

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Publications Citing Use of MedChemExpress (MCE) 1-Methylinosine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

1-Methylinosine Related Classifications

HY-113139 Related Classifications