7-Methylguanosine
Based on 2 publication(s) in Google Scholar
7-Methylguanosine is a modified nucleoside widely present in various RNAs and a key metabolite of the 5'-cap structure of eukaryotic mRNA. 7-Methylguanosine plays important roles in stabilizing RNA structures, regulating translation, and other aspects.
For research use only. We do not sell to patients.
- Purity: 99.10%
- CAS No.: 20244-86-4
- Formula: C11H16N5O5+
- Molecular Weight:298.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 7-Methylguanosine
MoreAll Endogenous Metabolite Isoforms
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Among the tRNA modifications, the 7-methylguanosine (m7G) modification is found widely in eubacteria, eukaryotes, and a few archaea. In most cases, the m7G modification occurs at position 46 in the variable region and is a product of tRNA (m7G46) methyltransferase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 20244-86-4
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Appearance Solid
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Molecular Weight 298.27
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Formula C11H16N5O5+
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Color White to light yellow
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SMILES
O=C1C2=C(N([C@H]3[C@H](O)[C@H](O)[C@@H](CO)O3)C=[N+]2C)NC(N)=N1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
H2O : 25 mg/mL (83.82 mM; ultrasonic and warming and heat to 60°C)
DMSO : 12.5 mg/mL (41.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (4.19 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (4.19 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 8.33 mg/mL (27.93 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Tomikawa C. 7-Methylguanosine Modifications in Transfer RNA (tRNA). Int J Mol Sci. 2018 Dec 17;19(12):4080. [Content Brief]
[2]. Kozarski M, et al. 7-Methylguanosine monophosphate analogues with 5'-(1,2,3-triazoyl) moiety: Synthesis and evaluation as the inhibitors of cNIIIB nucleotidase. Bioorg Med Chem. 2018 Jan 1;26(1):191-199. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.3527 mL | 16.7633 mL | 33.5267 mL | 83.8167 mL |
| 5 mM | 0.6705 mL | 3.3527 mL | 6.7053 mL | 16.7633 mL | |
| 10 mM | 0.3353 mL | 1.6763 mL | 3.3527 mL | 8.3817 mL | |
| 15 mM | 0.2235 mL | 1.1176 mL | 2.2351 mL | 5.5878 mL | |
| 20 mM | 0.1676 mL | 0.8382 mL | 1.6763 mL | 4.1908 mL | |
| 25 mM | 0.1341 mL | 0.6705 mL | 1.3411 mL | 3.3527 mL | |
| 30 mM | 0.1118 mL | 0.5588 mL | 1.1176 mL | 2.7939 mL | |
| 40 mM | 0.0838 mL | 0.4191 mL | 0.8382 mL | 2.0954 mL | |
| H2O | 50 mM | 0.0671 mL | 0.3353 mL | 0.6705 mL | 1.6763 mL |
| 60 mM | 0.0559 mL | 0.2794 mL | 0.5588 mL | 1.3969 mL | |
| 80 mM | 0.0419 mL | 0.2095 mL | 0.4191 mL | 1.0477 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
7-Methylguanosine Related Classifications
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