1. Academic Validation
  2. HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation

HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation

  • Oxid Med Cell Longev. 2022 Feb 7;2022:9030771. doi: 10.1155/2022/9030771.
Yuqin Ye 1 2 3 Zhusheng Feng 4 Shilai Tian 1 5 Yongxiang Yang 1 6 Yibin Jia 1 Guanyi Wang 1 Jiayou Wang 1 Wei Bai 1 Jinsheng Li 2 Xiaosheng He 1
Affiliations

Affiliations

  • 1 Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an 710032, China.
  • 2 Department of Aerospace Medicine, Air Force Medical University, Xi'an 710032, China.
  • 3 Department of Neurosurgery, PLA 921st Hospital, Changsha 410000, China.
  • 4 Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 710032, China.
  • 5 Department of Neurosurgery, The First Hospital of Lan Zhou University, Lan Zhou 730099, China.
  • 6 Department of Neurosurgery, The General Hospital of Western Theater Command, Chengdu 610083, China.
Abstract

Due to the limited neurogenesis capacity, there has been a big challenge in better recovery from neurological dysfunction caused by stroke for a long time. Neural stem cell (NSC) programmed death is one of the unfavorable factors for neural regeneration after stroke. The types of death such as Apoptosis and Necroptosis have been deeply investigated while the Pyroptosis of NSCs is not quite understood. Although it is well accepted that hyperbaric oxygen (HBO) alleviates the oxygen-glucose deprivation (OGD) injury after stroke and reduces programmed death of NSCs, whether NSC Pyroptosis is involved in this process is still unknown. Therefore, this study is aimed at studying the potential effect of HBO treatment on NSC Pyroptosis following OGD exposure, as well as its influence on NSC proliferation and differentiation in vitro. The results revealed that OGD increased NOD-like receptor protein 3 (NLRP3) expression to induce the pyroptotic death of NSCs, which was rescued by HBO treatment. And the upregulated lncRNA-H19 functioned as a molecular Sponge of miR-423-5p to target NLRP3 for NSC Pyroptosis following OGD. Most importantly, it was confirmed that HBO exerted protection of NSCs against Pyroptosis by inhibiting lncRNA-H19/miR-423-5p/NLRP3 axis. Moreover, HBO restraint of lncRNA-H19-associated Pyroptosis benefited the proliferation and neuronal differentiation of NSCs. It was concluded that HBO attenuated NSC Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 axis and enhanced neurogenesis following OGD. The findings provide new insight into NSC programmed death and enlighten therapeutic strategy after stroke.

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