Recognition and movement of polystyrene nanoplastics in fish cells

Although nanoplastics are being increasingly scrutinized, little is known about their kinetic behavior in living organisms, especially in cellular systems. Herein, nonspecific interactions of three polystyrene nanoplastics (pristine-PS, NH₂-PS, and COOH-PS, with size range of 90-100 nm and at concentrations of 0-100 μg mL^-1) with zebrafish cells were quantified for their cellular uptake and exocytosis. Cell uptake of nanoplastics reached a peak within 2 h and then decreased. The overall nanoplastics uptake was dominated by PS-particle internalization. The estimated uptake rate was comparable among the different types of PS (pristine-PS, NH₂-PS, and COOH-PS), but the uptake capacity was related to their functionality. The clathrin-mediated and caveolae-mediated pathways were mainly involved in the uptake of the three nanoplastics. The internalized PS-particles were initially delivered to the cytoplasm but then transported to lysosomes using energy. Meanwhile, these PS particles were released by the cells via energy-free penetration and energy-dependent lysosomal exocytosis. PS-particles were removed by the cells at a relatively slow rate, and the estimated retention half-lives of these PS-particles were 10.1 h, 12.0 h and 15.1 h for pristine-PS, NH₂-PS and COOH-PS particles, respectively, in fish cells based on our kinetic measurements. Intracellular trajectory modeling of nanoplastics movement is critical for the environmental and human health risk assessment.

Endocytosis; Exocytosis; Nanoplastics; Passive membrane penetration; Uptake.