2. Anti-infection
  3. Fungal Antibiotic
  4. Filipin complex

Filipin complex is a potent polyene macrolide antifungal antibiotic. Filipin complex inserts into membranes and sequester cholesterol into complexes and inhibits PRRSV entry. The Filipin complex consists of about 75.8% Filipin III (HY-N6718), 10.8% Filipin IV, 9.1% Filipin II, and 1.2% Filipin I (Ex/Em = 380/430 nm).

For research use only. We do not sell to patients.

CAS No. : 11078-21-0

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 47 publication(s) in Google Scholar

Other Forms of Filipin complex:

Filipin complex Related Antibodies

Top Publications Citing Use of Products

47 Publications Citing Use of MCE Filipin complex

IF
Cell Imaging/Staining
Flow Cytometry

    Filipin complex purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 May 23;10(1):171.  [Abstract]

    Flow cytometry analyses of the ox-LDL uptake in macrophages pretreated with Dynasore (10 mM), Filipin (5 μM; 30 min), or LY294002 (50 μM) and inoculated with/without Pg (MOI = 100). n = 4 per group.

    Filipin complex purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151.  [Abstract]

    Confocal micrographs of CD63 and filipin staining in cells transfected with Flag-V, Flag-GP73, or Flag-DM and quantitative mean fluorescence intensity (MFI) analysis of the Filipin, Filipin in MVBs, CD63. Green, GP73-GFP; red, CD63-mCherry; cyan, Filipin staining.

    Filipin complex purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Nov 14:57:344-357.  [Abstract]

    Representative confocal microscopy images showing intracellular localization of fluorescently labeled TQC (red) in HFSCs and DPCs after treatment with various endocytic inhibitors. Factin in green, nuclei in blue. Scale bar: 20 μm. Inhibitor concentrations: Filipin complex (20 μM;pre-incubated for 1 h), Methyl-β-cyclodextrin (100 μM), Dynasore (20 μM), Chlorpromazine hydrochloride (50 μM), Cytochalasin D (10 μg/mL), Amiloride hydrochloride (20 μM), Nocodazole (20 μM).

    Filipin complex purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 19;16(1):10123.  [Abstract]

    Filipin staining (50 μg/mL; 30 min in room temperature) to detect cholesterol from humans with OA (n = 6 per group) and from mice with OA (n = 4 per group).

    Filipin complex purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2022 Jun 13;7(1):176.  [Abstract]

    Representative images of different chCOs on D45 stained with Filipin (0.05  mg/ml in PBS containing 10% FBS for 2 h), MAP2, and human nuclei. Scale bar: 50 µm.

    View All Antibiotic Isoform Specific Products:

    View All Isoforms
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Filipin complex is a potent polyene macrolide antifungal antibiotic. Filipin complex inserts into membranes and sequester cholesterol into complexes and inhibits PRRSV entry. The Filipin complex consists of about 75.8% Filipin III (HY-N6718), 10.8% Filipin IV, 9.1% Filipin II, and 1.2% Filipin I (Ex/Em = 380/430 nm)[1][2][3].

    IC50 & Target

    Macrolide

     

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Solution preparation[4]
    1.1 Preparation of storage solution
    Solvent: DMSO
    Concentration: 50 mg/mL is recommended (e.g. weigh 50 mg Filipin complex and dissolve in 1 mL solvent).
    Storage: After aliquoting, store at -20°C or -80°C away from light and avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 50 μg/mL with PBS or serum-free cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately, and protected from light.

    2. For neuronal cholesterol levels staining.
    2.1 The staining sections are washed three times with PBS and incubated with 1.5 mg/mL glycine for 10 min at room temperature.
    2.2 Then sections are stained with 0.05 mg/mL Filipin complex in PBS containing 10% FBS for 2 h and rinsed for three times with PBS.
    2.3 Use an Olympus FV10i confocal microscope for image (Ex/Em = 380/430 nm).
    Filipin complex (2 μg/mL; 1+48 h) inhibits PRRSV entry with respiratory syndrome virus (PRRSV) genome copies in infected cells reduced by 61% in MARC-145 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Filipin complex Related Antibodies
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    Emission (Em)

    430
    Excitation (Ex)

    380
    SMILES

    CCCCC[C@@H]([R1])[C@]1([H])[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@H](O)/C(C)=C/C=C/C=C/C=C/C=C/[C@H]([R2])[C@@H](C)OC1=O.[Filipin I :R1=H,R2=H 1% Filipin II :R1=H,R2=OH 9% Filipin III :R1=(R)-OH,R2=OH 76% Filipin IV :R1=(S)-OH,R2=OH 11%]
    Structure Classification
    Initial Source

    Streptomyces filipinensis
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL

    Ethanol : 10 mg/mL (ultrasonic and warming and heat to 60°C)

    *"≥" means soluble, but saturation unknown.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 97.68%

    SDS (393 KB)

    COA (261 KB) RP-HPLC (2011 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Filipin complex Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Filipin complex11078-21-0FungalAntibioticCholesterolstainingfluorescentpolyeneantifungalantibioticmembrane sterolsInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Filipin complex
    Cat. No.:
    HY-N6716
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.