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  4. Filipin III

Filipin III is the major component of Filipin, a 28-membered ring pentaene macrolide antifungal antibiotic produced by S. filipinensis, S. avermitilis and S. miharaensis. Filipin interacts with membrane sterols causing the alteration of membrane structure.

For research use only. We do not sell to patients.

CAS No. : 480-49-9

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Based on 24 publication(s) in Google Scholar

Filipin III Related Antibodies

Top Publications Citing Use of Products

    Filipin III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Nov 13.

    Uptake of Liposomes by bEnd.3 Cells. Cells were pre-incubated at 4℃ or treated with Filipin III (1 μg/mL) for 1 hour. DiI-labeled liposomes were then added to the culture wells and incubated for an additional 2 h. Cell fluorescence intensity was measured using flow cytometry.

    Filipin III purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Jul 26;44(1):220.  [Abstract]

    Filipin III (0.05 mg/mL; 2 h). Filipin III staining displaying the cellular total cholesterol level in KYSE180 and KYSE30 cells with TTPAL depletion.

    Filipin III purchased from MedChemExpress. Usage Cited in: VIEW. 2025 Mar 23.

    Filipin III (100 µg/mL; 3 h). The Filipin III staining of BT549 and DU145 cells transduced with shSCR or shSOAT1.

    Filipin III purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 29;26(7):3172.  [Abstract]

    The pathways of D-M159 entry into cells were determined using 5 µg/mL Filipin III.

    Filipin III purchased from MedChemExpress. Usage Cited in: Mol Pharm. 2024 May 9.  [Abstract]

    Fluorescence detection of cholesterol. HeLa cells were incubated with different concentrations of QS-21 (2.5, 5, and 7.5 μM) for 12 h, followed by staining with Filipin III (30 min). Images were captured by fluorescence microscopy.

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    Description

    Filipin III is the major component of Filipin, a 28-membered ring pentaene macrolide antifungal antibiotic produced by S. filipinensis, S. avermitilis and S. miharaensis. Filipin interacts with membrane sterols causing the alteration of membrane structure[1].

    IC50 & Target

    Macrolide

     

    In Vitro

    Cell staining:
    Prepare Filipin III working solution in TBS-T. NCI-60 cancer cells were treated with 50 μg/mL Filipin III in the dark. After incubation for 1 hour at room temperature, cells were washed with TBS-T. Coverslips were then detached and mounted in a water-based mountant. Images were taken on an Olympus Provis fluorescence microscope equipped with a 40X oil objective, and quantification of cholesterol levels was accomplished using the particle size algorithm in MetaXpress image analysis software (Molecular Devices Corp., Sunnyvale CA)[2].
    Huang Y, et al assessed cholesterol enrichment on transsomes relative to TSPAN-GFP. NRK cells stably expressing TSPAN4-GFP were incubated with 2 μg/mL Filipin III at 37 °C. After 15 minutes of treatment, Z-stack images were acquired using confocal microscopy to measure filipin III intensity on migrasomes (IpM), TSPAN4-GFP intensity on migrasomes (ItM), filipin III intensity on RFs (IpR), and TSPAN4- GFP on RF. Strength(ItR)[3].
    Or as Kwiatkowska K, et al exposed fibroblasts (Fibroblast) to 25 μg/ml Filipin III and incubated in the dark for 45 minutes (20 °C), the staining results were obtained[4].

    Tissue staining:
    Filipin binds to cholesterol with high affinity; however, it exhibits rapid photobleaching and produces only modest natural fluorescence upon UV excitation, making its detection under confocal microscopy difficult[5].
    Reid PC et al used Filipin III to detect cholesterol accumulation in neurons. NPC cerebellar tissue was stained with Filipin III (125 μg/ml) for 2 h at room temperature in the dark. After incubation together, slides were washed with 0.5 M Tris without FBS and counterstained with fluorescent dyes. On day 22, cholesterol accumulation was observed in Purkinje neuronal cells. Or staining the NPC cortex with Filipin III, cholesterol accumulation in the axon hillock region of most neurons was observed on day 22[5].
    Novak A et al use Filipin III to detect cholesterol accumulation in tumors. Dissolve Filipin III in DMSO/PBS (1:4) to make a working solution of 50 μg/mL. Subsequently, the tissue sections were incubated at room temperature for 30 min. After rinsing, the slides were mounted in an aqueous fluorescent mountant for subsequent observation[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Filipin III Related Antibodies
    Molecular Weight

    654.83

    Formula

    C35H58O11
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1O[C@H](C)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@H]1[C@H](O)CCCCC
    Structure Classification
    Initial Source

    Streptomyces miharaensis
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 10 mg/mL (15.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Methanol : 10 mg/mL (15.27 mM; Need ultrasonic and warming)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5271 mL 7.6356 mL 15.2711 mL
    5 mM 0.3054 mL 1.5271 mL 3.0542 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.0%

    SDS (394 KB)

    COA (255 KB) HNMR (312 KB) RP-HPLC (128 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Methanol 1 mM 1.5271 mL 7.6356 mL 15.2711 mL 38.1778 mL
    5 mM 0.3054 mL 1.5271 mL 3.0542 mL 7.6356 mL
    10 mM 0.1527 mL 0.7636 mL 1.5271 mL 3.8178 mL
    15 mM 0.1018 mL 0.5090 mL 1.0181 mL 2.5452 mL
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    Filipin III Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Filipin III480-49-9AntibioticFungalInhibitorinhibitorinhibit

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