Filipin complex (solution)

Guidelines (The following are our recommended protocols. This protocol is for reference only and should be modified according to your specific needs.)
1. Solution preparation^[4]
1.1 Preparation of working solution
Dilute to 50 μg/mL with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately, and protected from light.
The stock soultion, store at -20°C or -80°C away from light and avoid repeated freezing and thawing.

For neuronal cholesterol levels staining.
1. The staining sections are washed three times with PBS and incubated with 1.5 mg/mL glycine for 10 min at room temperature.
2. Then sections are stained with 50 μg/mL Filipin complex in PBS containing 10% FBS for 2 h and rinsed for three times with PBS.
3. Use an Olympus FV10i confocal microscope for image (Ex/Em = 380/430 nm).

Filipin complex (2 μg/mL; 1+48 h) inhibits PRRSV entry with respiratory syndrome virus (PRRSV) genome copies in infected cells reduced by 61% in MARC-145 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.