2. Academic Validation
  3. Evaluation of the antibacterial activity of hecogenin acetate and its inhibitory potential of NorA and MepA efflux pumps from Staphylococcus aureus

Evaluation of the antibacterial activity of hecogenin acetate and its inhibitory potential of NorA and MepA efflux pumps from Staphylococcus aureus

  • Microb Pathog. 2023 Jan:174:105925. doi: 10.1016/j.micpath.2022.105925.
Nara Juliana Santos Araújo 1,  Ana Raquel Pereira da Silva 2,  Maria do Socorro Costa 2,  Camila Aparecida Pereira Silva 3,  Thiago Sampaio de Freitas 4,  Erlanio Oliveira de Sousa 5,  José Maria Barbosa Filho 6,  Yedda Maria Lobo Soares de Matos 4,  Henrique Douglas Melo Coutinho 7,  Jacqueline Cosmo Andrade-Pinheiro 8
Affiliations

Affiliations

  • 1 Graduate Program in Health Sciences, Federal University of Cariri, 63180-000, Barbalha, Ceara, Brazil; Laboratory Applied Microbiology - LAMAP, Federal University of Cariri - UFCA, R. Olegário Emidio de Araujo, s/n, CEP 63260-000, Brejo Santo, CE, Brazil.
  • 2 Graduate Program in Biotechnology, State University of Ceara, Fortaleza, Ceara, Brazil; Laboratory of Microbiology and Molecular Biology, Regional University of Cariri - LMBM, Crato, Ceara, Brazil.
  • 3 Laboratory Applied Microbiology - LAMAP, Federal University of Cariri - UFCA, R. Olegário Emidio de Araujo, s/n, CEP 63260-000, Brejo Santo, CE, Brazil.
  • 4 Laboratory of Microbiology and Molecular Biology, Regional University of Cariri - LMBM, Crato, Ceara, Brazil.
  • 5 Faculty of Technology of Cariri - FATEC, Juazeiro do Norte, Ceara, Brazil.
  • 6 Laboratory Technology Pharmaceutical, Federal University of Paraíba, Joao Pessoa, Paraíba, Brazil.
  • 7 Laboratory of Microbiology and Molecular Biology, Regional University of Cariri - LMBM, Crato, Ceara, Brazil. Electronic address: [email protected].
  • 8 Graduate Program in Health Sciences, Federal University of Cariri, 63180-000, Barbalha, Ceara, Brazil; Laboratory Applied Microbiology - LAMAP, Federal University of Cariri - UFCA, R. Olegário Emidio de Araujo, s/n, CEP 63260-000, Brejo Santo, CE, Brazil. Electronic address: [email protected].
PMID: 36462578 DOI: 10.1016/j.micpath.2022.105925
Abstract

Antimicrobial drugs are of great importance in the control of Bacterial infections. Its indiscriminate use contributes to the consolidation of Bacterial resistance. Its applicability is due to its secondary metabolites, such as saponins, which are compounds with relevant Antibacterial action. Hecogenin acetate is a saponin present in Plants of the agave genus with analgesic, antioxidant, antinociceptive, cardioactive, Anticancer, Antifungal and antimicrobial activity. The present work aimed to identify the Antibacterial activity of hecogenin acetate against strains of E. coli, P. aeruginosa and S. aureus and to investigate the NorA and MepA efflux pump inhibitory activity of S. aureus strains. The Minimum Inhibitory Concentration was evaluated by broth microdilution. The Antibiotic Activity Modifier effect and the assessment of efflux pump inhibition were evaluated by microdilution with sub-inhibitory concentrations. Hecogenin acetate showed minimal inhibitory concentration without significant relevance. In the evaluation of the potentiating activity of the Antibiotic action, a greater antagonistic behavior is noticed. In the analyzes performed with the efflux pump, it was noticed that the hecogenin acetate does not interfere in the efflux pump mechanism of the analyzed bacteria.

Keywords

Efflux inhibitor; Resistance; Saponin.

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