Hecogenin acetate
Based on 1 Customer Validation
Hecogenin acetate is an orally active steroid saponin aglycone with extensive biological activities. Hecogenin acetate inhibits the phosphorylation of NF-κB and p38 MAPK signaling pathways, antagonizes TRPA1/TRPM8 channels, inhibits the production of pro-inflammatory cytokines, and has anti-inflammatory and analgesic effects. Hecogenin acetate inhibits the production of ROS and the activation of NLRP3 inflammasome; downregulates the expression of MMP-2, and has neuroprotective and anti-tumor activities. Hecogenin acetate enhances gastric mucosal defense and promotes ulcer healing. Hecogenin acetate can be used in combination with certain antibiotics to regulate bacterial efflux pumps and restore antibiotic sensitivity.
For research use only. We do not sell to patients.
- Purity: 98.57%
- CAS No.: 915-35-5
- Formula: C29H44O5
- Molecular Weight:472.66
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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p38 MAPK |
NF-κB |
ERK1 |
ERK2 |
MMP-2 |
NLRP3 inflammasome |
IL-1β |
IL-6 |
Hecogenin acetate has only a very weak direct antibacterial activity with MIC values for S. aureus ATCC and E. coli ATCC of 645 μg/mL and 512 μg/mL respectively[3].
Hecogenin acetate (1-150 μM, 24 h) has no significant effect on the viability of A549 cells when the concentration was lower than 75 μM, but at higher concentrations it reduces the viability of A549 cells[4].
Hecogenin acetate (50 μM, 24 h) reverses the cell membrane damage, ROS production, and increased activities of catalase (CAT) and superoxide dismutase (SOD) caused by H₂O₂ in A549 cells[4].
Hecogenin acetate (25-100 μM, 24-72 h) induces G0/G1 phase cell cycle arrest and cellular senescence by inhibiting the ERK1/2 signaling pathway and reducing the expression of MMP-2[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 cells
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Concentration:25, 50, 75 and 100 μM
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Incubation Time:72 h
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Result:Dose-dependently arrested A549 cells in the G0/G1 phase.
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Cell Line:A549 cells
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Concentration:50 μM
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Incubation Time:24 h
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Result:Completely blocked the ERK1/2 phosphorylation induced by H₂O₂.
Inhibited the upregulation of matrix metalloproteinase-2 (MMP-2) expression caused by H₂O₂.
Hecogenin acetate (5-25 mg/kg, i.p., single dose or once daily for 7 days) inhibits the expression of the aldosterone synthase (CYP11B2) gene, thereby exerting a diuretic effect in rats[2].
Hecogenin acetate (2.5-10 mg/kg, p.o., single dose or once daily for 7 days) exhibits significant protective effects against acute gastric mucosal injury induced by various chemical substances (anhydrous ethanol, acidified ethanol) and pathological processes (ischemia-reperfusion) in rodent models[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Gastric injury induced by ischemia-reperfusion established in 2-month male Swiss mice weighing between 25 and 30 g[5]
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Dosage:20 mg/kg
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Administration:Intragastric administration (i.g.), once daily for 8 days (chronic pain model) or 15 days (neuropathic pain model model)
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Result:Exhibited potent and long-lasting anti-allodynic effects in both chronic and neuropathic pain models.
Inhibited the activation of spinal microglia, reduce the release of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and inhibit the activation of the NF-κB and p38 MAPK signaling pathways.
Antagonized TRPM8 and TRPA1 ion channels, reducing the sensitization of peripheral nociceptive receptors.
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Animal Model:Acute and subacute diuretic assays established in Sprague-Dawley rats (180-240 g)[2]
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Dosage:5, 10 and 25 mg/kg
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Administration:Intraperitoneal injection (i.p.), single dose or once daily for 7 days
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Result:Had a dose-dependent diuretic effect, which can significantly increase urine output and the excretion of sodium and chloride ions. Showed the characteristics of sodium and chlorine excretion while maintaining relatively high potassium levels. Down-regulated the expression of the aldosterone synthase (CYP11B2) gene in the adrenal gland to inhibit aldosterone synthesis.
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Animal Model:Acute gastric ulcers induced by anhydrous ethanol and acidified ethanol established in 2-month male Swiss mice eighing between 25 and 30 g[5]
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Dosage:5, 10, 20 mg/kg
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Administration:Oral administration (p.o.), single dose
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Result:Had a strong protective effect against acute gastric mucosal injury caused by anhydrous ethanol.
Effectively resisted more severe damage to the gastric mucosa caused by the combined attack of ethanol and gastric acid.
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Animal Model:Gastric injury induced by ischemia-reperfusion and chronic gastric ulcers induced by acetic acid established in male Sprague-Dawley rats (250-300 g)[5]
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Dosage:5, 10, 20 mg/kg
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Administration:Oral administration (p.o.), single dose (gastric injury model) or once daily for 7 days (chronic gastric ulcers mmodel)
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Result:Had a strong protective effect against ischemia-reperfusion gastric injury mediated by oxidative stress.
Effectively promoted the healing of chronic gastric ulcers.
Chemical Information
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CAS No. 915-35-5
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Appearance Solid
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Molecular Weight 472.66
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Formula C29H44O5
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Color White to yellow
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SMILES
[H][C@]1(O[C@@]2(OC[C@H](C)CC2)[C@@H](C)[C@@]1([C@]34C)[H])C[C@@]3([H])[C@]5([H])CC[C@@]6([H])C[C@@H](OC(C)=O)CC[C@]6(C)[C@@]5([H])CC4=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
THF : 57.5 mg/mL (121.65 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (285 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Santos Passos FR, et al. Role of peripheral and central sensitization in the anti-hyperalgesic effect of hecogenin acetate, an acetylated sapogenin, complexed with β-cyclodextrin: Involvement of NFκB and p38 MAPK pathways. Neuropharmacology. 2021 Mar 15;186:108395. [Content Brief]
[2]. Alamoudi AJ, et al. Diuretic effects of Hecogenin and Hecogenin acetate via aldosterone synthase inhibition. Saudi Pharm J. 2024 Jul;32(7):102105. [Content Brief]
[3]. Santos Araújo NJ, et al. Evaluation of the antibacterial activity of hecogenin acetate and its inhibitory potential of NorA and MepA efflux pumps from Staphylococcus aureus. Microb Pathog. 2023 Jan;174:105925. [Content Brief]
[4]. Gasparotto J, et al. Hecogenin acetate inhibits reactive oxygen species production and induces cell cycle arrest and senescence in the A549 human lung cancer cell line. Anticancer Agents Med Chem. 2014;14(8):1128-35. [Content Brief]
[5]. Sousa AJC, et al. Antiulcerogenic and healing activity of hecogenin acetate in rodents. Naunyn Schmiedebergs Arch Pharmacol. 2023 Apr;396(4):759-769. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| THF | 1 mM | 2.1157 mL | 10.5784 mL | 21.1569 mL | 52.8921 mL |
| 5 mM | 0.4231 mL | 2.1157 mL | 4.2314 mL | 10.5784 mL | |
| 10 mM | 0.2116 mL | 1.0578 mL | 2.1157 mL | 5.2892 mL | |
| 15 mM | 0.1410 mL | 0.7052 mL | 1.4105 mL | 3.5261 mL | |
| 20 mM | 0.1058 mL | 0.5289 mL | 1.0578 mL | 2.6446 mL | |
| 25 mM | 0.0846 mL | 0.4231 mL | 0.8463 mL | 2.1157 mL | |
| 30 mM | 0.0705 mL | 0.3526 mL | 0.7052 mL | 1.7631 mL | |
| 40 mM | 0.0529 mL | 0.2645 mL | 0.5289 mL | 1.3223 mL | |
| 50 mM | 0.0423 mL | 0.2116 mL | 0.4231 mL | 1.0578 mL | |
| 60 mM | 0.0353 mL | 0.1763 mL | 0.3526 mL | 0.8815 mL | |
| 80 mM | 0.0264 mL | 0.1322 mL | 0.2645 mL | 0.6612 mL | |
| 100 mM | 0.0212 mL | 0.1058 mL | 0.2116 mL | 0.5289 mL |
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