2. Academic Validation
  3. BMP9 promotes autophagy and inhibits migration and invasion in breast cancer cells through the c-Myc/SNHG3/mTOR signaling axis

BMP9 promotes autophagy and inhibits migration and invasion in breast cancer cells through the c-Myc/SNHG3/mTOR signaling axis

  • Tissue Cell. 2023 Jun:82:102073. doi: 10.1016/j.tice.2023.102073.
Huomei Yu 1,  Yuanxiang Chen 1,  Lei Lang 2,  Deyu Liao 1,  Shiyan Liu 1,  Tao Yu 1,  Kai Hu 1,  Lan Zhou 1,  Yan Zhang 3
Affiliations

Affiliations

  • 1 Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
  • 2 Department of Clinical Laboratory, Chongqing Emergency Medical Center, Chongqing University Central Hospital, School of Medicine, Chongqing University, Chongqing 400014, PR China.
  • 3 Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: [email protected].
PMID: 36963166 DOI: 10.1016/j.tice.2023.102073
Abstract

We previously reported that BMP9 inhibited breast Cancer progression. However, the precise molecular mechanism is still unknown. Based on our RNA-sequencing (RNA-seq) results, BMP9 significantly down-regulated the expression of long non-coding RNA SNHG3. Exogenous BMP9 promoted Autophagy and inhibited migration and invasion in MDA-MB-231 cells, which was effectively blunted by SNHG3 overexpression. Interestingly, SNHG3 was negatively connected with Autophagy. Knockdown of SNHG3 induced Autophagy by increasing the formation of autophagic vacuoles and thus inhibited the migration and invasion of MDA-MB-231 cells. Mechanically, BMP9-SNHG3 activated AMPK, Akt and mTOR signaling pathways to induce Autophagy and inhibit migration and invasion. Meanwhile, BMP9 regulated SNHG3 transcription by suppressing c-Myc entry into the nucleus. In conclusion, BMP9 promotes Autophagy and inhibits migration and invasion in breast Cancer cells through the c-Myc/SNHG3/mTOR signaling axis, which might offer a fresh perspective on BMP9's breast cancer-inhibiting properties.

Keywords

Autophagy; BMP9; Breast cancer; Invasion; Long non-coding RNA; Migration.

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