2. Academic Validation
  3. SOV sensitizes gastric cancer cells to radiation by suppressing radiation-induced autophagy in vitro and in vivo

SOV sensitizes gastric cancer cells to radiation by suppressing radiation-induced autophagy in vitro and in vivo

  • Tissue Cell. 2023 Jun:82:102109. doi: 10.1016/j.tice.2023.102109.
Wen Wang 1,  Xiao-Gan Jiang 2,  Ya-Ping Bai 3,  Heng Li 4,  Ling-Xi Gao 1,  Teng Zhang 1,  Fang-Yi Dong 5,  Wen-Ping Ding 6,  Yan Zhang 7
Affiliations

Affiliations

  • 1 Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu 241002, China; Department of Gastroenterology, The First Aflliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China.
  • 2 Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu 241002, China; Department of Critical Care Medicine, The First Aflliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China.
  • 3 Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu 241002, China; College of Life Sciences, Anhui Normal University, Wuhu, 241000, China.
  • 4 General Sugery Depatment, Shanghai Baoshan District Hospital of Integrated Traditional and Western Medicine, Shanghai 201900, China.
  • 5 Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: [email protected].
  • 6 Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu 241002, China; Department of Radiotherapy, The First Aflliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China. Electronic address: [email protected].
  • 7 Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu 241002, China; Department of Gastroenterology, The First Aflliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China. Electronic address: [email protected].
PMID: 37229935 DOI: 10.1016/j.tice.2023.102109
Abstract

Vanadium is a transition metal that naturally occurs in the environment and has a variety of biological and physiological impacts on humans. Sodium orthovanadate (SOV), a well-known chemical compound of vanadium, has shown notable anti-cancer activity in various types of human malignancies. However, the effect of SOV on stomach Cancer is yet undetermined. Furthermore, only a few studies have investigated the association of SOV and radiosensitivity with stomach Cancer. Our study has investigated the ability of SOV to increase the sensitivity of gastric Cancer cells to radiation. To detect Autophagy triggered by ionizing radiation and the influence of SOV on cell radiosensitivity, the Cell Counting Kit-8 (CCK8) test, EDU staining experiment, colony formation assay, and immunofluorescence were performed. The possible synergistic effects of SOV and irradiation were examined in vivo using a xenograft mouse model of stomach Cancer cells. Both in vitro and in vivo studies showed that SOV markedly reduced the growth of stomach Cancer cells and improved their radiosensitivity. Our results showed that SOV increased gastric Cancer cells' radiosensitivity, thereby blocking the radiation-induced autophagy-related protein, ATG10. Thus, SOV can be considered a potential agent for radiosensitizing gastric Cancer.

Keywords

ATG10; Autophagy; Gastric cancer; Radiosensitivity; SOV.

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