2. Academic Validation
  3. Autophagy is involved in degradation of AQP1 in response to an acute decrement in tonicity

Autophagy is involved in degradation of AQP1 in response to an acute decrement in tonicity

  • iScience. 2023 Nov 20;26(12):108485. doi: 10.1016/j.isci.2023.108485.
Xiangdong Guo 1 2,  Long Xu 1 2,  Yonglun Kong 2 3,  Meng Li 1 2,  Qingxian Zhai 2 3,  Baien Liang 2 3,  Xiaoduo Zhao 2 3,  Luosha Long 1 2,  Meiying Huang 2 3,  Weidong Wang 2 3,  Chunling Li 1 2
Affiliations

Affiliations

  • 1 Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
  • 2 Institute of Hypertension, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
  • 3 Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
PMID: 38094243 DOI: 10.1016/j.isci.2023.108485
Abstract

Renal medullary aquaporin-1 (AQP1) plays an important role in the urinary concentration. This study aimed to investigate the regulation of AQP1 by low osmotic stress and a potential role of Autophagy. Low osmotic stress induced a dramatically decreased AQP1 protein expression in murine inner medullary collecting duct 3 (mIMCD3) cells, which was associated with a marked activation of Autophagy. Inhibition of Autophagy by 3-methyladenine (3-MA), chloroquine, or knockdown of autophagy-related protein 5 (ATG5) prevented the decrease in AQP1 protein abundance. Rapamycin-induced Autophagy was associated with a decreased AQP1 protein expression and an enhanced interaction between AQP1 and ATG5 in mIMCD3 cells under low osmotic stress. In kidney inner medulla of mice given a 3% NaCl solution, activation of Autophagy was associated with decreased AQP1 protein expression, which was prevented by 3-MA. In conclusion, low osmotic stress induced Autophagy which contributed to the decreased AQP1 protein expression in the renal medulla.

Keywords

Biological sciences; Cell biology; Molecular biology.

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