2. Academic Validation
  3. Platelet membrane-derived biomimetic microbubbles with enhanced targeting ability for the early detection of myocardial ischemia-reperfusion injury

Platelet membrane-derived biomimetic microbubbles with enhanced targeting ability for the early detection of myocardial ischemia-reperfusion injury

  • Colloids Surf B Biointerfaces. 2024 Feb:234:113680. doi: 10.1016/j.colsurfb.2023.113680.
Ying Bai 1,  Yihan Chen 2,  Qiaofeng Jin 1,  Cheng Deng 1,  Lingling Xu 1,  Tian Huang 1,  Shukun He 1,  Yanan Fu 1,  Jiani Qiu 1,  Jia Xu 1,  Tang Gao 1,  Wenqian Wu 1,  Qing Lv 1,  Yali Yang 1,  Li Zhang 3,  Mingxing Xie 3,  Xiaoqiu Dong 4,  Jing Wang 5
Affiliations

Affiliations

  • 1 Department of Ultrasound Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Clinical Research Center for Medical Imaging in Hubei Province, China; Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China.
  • 2 Department of Ultrasound Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Clinical Research Center for Medical Imaging in Hubei Province, China; Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China; Department of Ultrasonography, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
  • 3 Department of Ultrasound Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Clinical Research Center for Medical Imaging in Hubei Province, China; Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China; Shenzhen Huazhong University of Science and Technology Research Institute, China.
  • 4 Department of Ultrasonography, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: [email protected].
  • 5 Department of Ultrasound Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Clinical Research Center for Medical Imaging in Hubei Province, China; Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China. Electronic address: [email protected].
PMID: 38101143 DOI: 10.1016/j.colsurfb.2023.113680
Abstract

Myocardial ischemia-reperfusion injury (MIRI) is a widely recognized Cardiovascular Disease that significantly impacts the prognosis of patients undergoing myocardial infarction recanalization. This condition can be fatal and involves complex pathophysiological mechanisms. Early diagnosis of MIRI is crucial to minimize myocardial damage and reducing mortality. Based on the inherent relationship between platelets and MIRI, we developed biomimetic microbubbles coated with platelet membrane (MB-pla) for early identification of MIRI. The MB-pla were prepared through a recombination process involving platelet membrane obtained from rat whole blood and Phospholipids, blended in appropriate proportions. By coating the microbubbles with platelet membrane, MB-pla acquired various adhesion molecules, thereby gaining the capability to selectively adhere to damaged endothelial cells in the context of MIRI. In vitro experiments demonstrated that MB-pla exhibited remarkable targeting characteristics, particularly toward type IV Collagen and human umbilical vein endothelial cells that had been injured through hypoxia/reoxygenation procedures. In a rat model of MIRI, the signal intensity produced by MB-pla was notably higher than that of control microbubbles. These findings were consistent with results obtained from fluorescence imaging of isolated hearts and immunofluorescence staining of tissue sections. In conclusion, MB-pla has great potential as a non-invasive early detection method for MIRI. Furthermore, this approach can potentially find application in Other conditions involving endothelial injury in the future.

Keywords

Microbubble; Molecular ultrasound imaging; Myocardial ischemia-reperfusion injury; Platelet membrane.

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