2. Academic Validation
  3. ACSL1 positively regulates adipogenic differentiation

ACSL1 positively regulates adipogenic differentiation

  • Biochem Biophys Res Commun. 2024 Nov 26:735:150865. doi: 10.1016/j.bbrc.2024.150865.
Yao Jiang 1,  Wei Wang 2,  Hui Wang 1,  Xiaoru Zhang 1,  Yuling Kong 1,  Yong Q Chen 3,  Shenglong Zhu 4
Affiliations

Affiliations

  • 1 Wuxi School of Medicine, Jiangnan University, Wuxi, 214000, China.
  • 2 Wuxi School of Medicine, Jiangnan University, Wuxi, 214000, China; The Second School of Clinical Medical, Xuzhou Medical University, Xuzhou, Jiangsu, China.
  • 3 Wuxi School of Medicine, Jiangnan University, Wuxi, 214000, China; MOE Medical Basic Research Innovation Center for Gut Microbiota and Chronic Diseases, School of Medicine, Jiangnan University, China. Electronic address: [email protected].
  • 4 Wuxi School of Medicine, Jiangnan University, Wuxi, 214000, China. Electronic address: [email protected].
PMID: 39442449 DOI: 10.1016/j.bbrc.2024.150865
Abstract

Aberrant adipogenic differentiation is strongly associated with obesity and related metabolic diseases. Elucidating the key factors driving adipogenesis is an effective strategy for identifying novel therapeutic targets for treating metabolic diseases represented by obesity. In this study, transcriptomic techniques were employed to investigate the functional genes that regulate adipogenic differentiation in OP9 cells and 3T3-L1 cells. The findings indicated a notable upregulation of Acsl1 expression throughout the adipogenic differentiation process. Knocking down Acsl1 led to a decrease in the expression of genes associated with adipogenesis and a reduction in triglyceride accumulation. Additionally, Acsl1 overexpression promoted adipocyte differentiation and adipose-specific overexpression of Acsl1 markedly aggravated steatosis induced by a high-fat diet. Mechanistically, Cyp2f2, Dusp23 and Gstm2 are the crucial genes implicated in Acsl1-induced adipogenic differentiation. The findings of this study indicate that Acsl1 promotes adipogenesis and could serve as a potential therapeutic target for treating obesity and related metabolic disorders.

Keywords

Acsl1; Adipogenesis; Adipogenic differentiation; Obesity.

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