2. Academic Validation
  3. Calpain-1 Potentiates Periodontal Regeneration via PHLPP1-ERK-Driven Osteogenesis in Periodontal Ligament Stem Cells

Calpain-1 Potentiates Periodontal Regeneration via PHLPP1-ERK-Driven Osteogenesis in Periodontal Ligament Stem Cells

  • Int Dent J. 2025 Dec 6;76(1):109309. doi: 10.1016/j.identj.2025.109309.
Yuejia Li 1,  Bin Yang 1,  Ziyi Mei 1,  Pingmeng Deng 1,  Tiwei Fu 1,  Jie Li 2
Affiliations

Affiliations

  • 1 College of Stomatology, Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China; Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering, Chongqing Medical University, Chongqing, China.
  • 2 College of Stomatology, Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China; Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering, Chongqing Medical University, Chongqing, China. Electronic address: [email protected].
PMID: 41353895 DOI: 10.1016/j.identj.2025.109309
Abstract

Objectives: In recent years, human periodontal ligament stem cells (hPDLSCs) are considered a promising seed cell source for bone tissue engineering, offering a prospective treatment for periodontal bone defects. The osteogenic differentiation of hPDLSCs is a critical process in bone tissue engineering. Calcium signalling regulates a myriad of cellular processes, including osteogenic differentiation; however, the function of calpain-1 (CAPN1), a calcium-dependent protein, remains incompletely elucidated.

Methods: Intracellular CA2+ levels were evaluated using Fluo-4 AM assay. CAPN1 was overexpressed by transfection of lentivirus. Knockdown of CAPN1 was performed using siRNA transfection. mRNA and protein expression were detected through Real-Time PCR and Western blot, respectively. Immunofluorescence assay was performed using anti-CAPN1 and anti-phosphatase PH domain and leucine-rich repeat protein Phosphatase 1 (PHLPP1) antibodies. Hydroxyapatite/beta-tricalcium phosphate loaded with CAPN1-overexpressing hPDLSCs were implanted into the dorsal region of nude mice. Periodontal bone defects were prepared using a trephine drill, and GelMA loaded with CAPN1-overexpressing hPDLSCs were implanted into the defect area.

Results: Fluo-4 AM assay showed a continuous upregulation of intracellular CA2+ levels, CAPN1 expression, and calpain activity during osteogenic induction of hPDLSCs. Overexpression of CAPN1 enhanced osteogenic differentiation of hPDLSCs and involved activation of the ERK1/2 signalling pathway. Knockdown CAPN1 or inhibiting CAPN1 by chemical inhibitor decreased osteogenic differentiation of hPDLSCs through ERK1/2 signalling pathway. Immunofluorescence staining and molecular docking analysis suggested that CAPN1 was colocalized and bound with PHLPP1. Furthermore, the combination of CAPN1-overexpressing hPDLSCs with hydroxyapatite/beta-tricalcium phosphate or GelMA hydrogel significantly enhanced bone regeneration in both subcutaneous implantation and periodontal bone defect models.

Conclusion: Our study reveals the role and mechanism of CAPN1 in the osteogenic differentiation of hPDLSCs, which provides a theoretical and technical foundation for targeting CAPN1 in bone regeneration.

Keywords

Bone tissue engineering; Calpain 1; ERK1/2; Osteogenic differentiation; hPDLSCs.

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