2. Metabolic Enzyme/Protease Apoptosis
  3. Cathepsin Proteasome Apoptosis
  4. Calpeptin

Calpeptine est un inhibiteur puissant de la calpaïne pénétrant dans les cellules, avec un ID50 de 40 nM pour la Calpain I dans les plaquettes humaines. Calpeptine est également un inhibiteur de la cathepsine K.

Calpeptin ist ein starker, zelldurchdringender calpain-Inhibitor mit einer ID50 von 40 nM für Calpain I in menschlichen Blutplättchen. Calpeptin ist auch ein Inhibitor von cathepsin K.

Calpeptin is a potent, cell penetrating calpain inhibitor, with an ID50 of 40 nM for Calpain I in human platelets. Calpeptin is also an inhibitor of cathepsin K.

For research use only. We do not sell to patients.

CAS No. : 117591-20-5

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Based on 27 publication(s) in Google Scholar

Other Forms of Calpeptin:

Calpeptin Related Antibodies

Top Publications Citing Use of Products

27 Publications Citing Use of MCE Calpeptin

Histological Imaging/Staining
IF
WB
In Vivo Efficacy Study

    Calpeptin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr 2:e2501112.  [Abstract]

    Calpeptin (0.01 mg/mL/g via intraperitoneal injection, administered three times per week, starting 3 days prior to BAPN treatment. Representative microscopic images of aorta sections stained with H&E and EVG (scale bar: 100 μm).

    Calpeptin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr 2:e2501112.  [Abstract]

    HAECs were treated with 1 μmol Angiotensin II (AngII) for 24 hours. The calpain inhibitor Calpeptin was administered at a concentration of 10 μmol for 1 hour prior to the Ang II treatment. Representative Western blot images for Calpain-2, Itgav, Itgb3, Talin1, Vinculin, and VE-cadherin are shown, withGAPDH serving as the loading control.

    Calpeptin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr 2:e2501112.  [Abstract]

    Pharmacological inhibition of calpain using Calpeptin (0.01 mg/mL/g via intraperitoneal injection, administered three times per week, starting 3 days prior to BAPN treatment.

    Calpeptin purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2022 Mar 28;20(1):165.  [Abstract]

    Cell immunofluorescence assay confirming the presence of NPM3-EGFP in recipient cells via the transwell assay, GW4869 or Calpeptin (50 nM, 48 h) was used to block sEVs release, scale bar=50 μm.

    Calpeptin purchased from MedChemExpress. Usage Cited in: ACS Cent Sci. 2022 Feb 23;8(2):192-204.

    CAPNS1 degradation was mediated by calpain activity. HEK293T cells transfected with HA or HA-CRBN for 36 hours were subsequently treated with dimethyl sulfoxide (DMSO), Calpeptin (10 μM), or MG-132 (10 μM) for an additional 12 hours, after which western blotting was performed.

    View All Cathepsin Isoform Specific Products:

    View All Isoforms
    Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Calpeptin is a potent, cell penetrating calpain inhibitor, with an ID50 of 40 nM for Calpain I in human platelets[1]. Calpeptin is also an inhibitor of cathepsin K[2].

    IC50 & Target

    cathepsin K

     

    Cellular Effect
    Cell Line Type Value Description References
    HeLa IC50
    17.7 μM
    Compound: 4
    Antiviral activity against human rhinovirus-14 in HeLa cells
    Antiviral activity against human rhinovirus-14 in HeLa cells
    		10.1016/S0960-894X(96)00537-9
    In Vitro

    Calpeptin (0-100 nM, 24 hours) treatment suppresses the proliferation of both WI38 VA13 and IMR90 cells in a dose-dependent manner. Calpeptin (1000 pg/ml, 24 hours) inhibits IL-6-induced cell proliferation of lung fibroblasts[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Calpeptin Related Antibodies

    Cell Proliferation Assay[3]

    Cell Line: WI38 VA13 and IMR90 cells
    Concentration: 0-100 nM
    Incubation Time: 24 hours
    Result: Suppressed the proliferation in a dose-dependent manner.
    In Vivo

    Calpeptin with Bleo (0.04 mg/mouse, i.p., 3 times weekly, 28 days) administration significantly inhibits the collagen deposition and increases of calpain activity in the NSC-125066 treated mouse lung tissues[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6 female mice (Eight-week-old)[3].
    Dosage: 0.04 mg/mouse.
    Administration: Intraperitoneally three times weekly for 28 days (together with Bleo).
    Result: Inhibited the collagen deposition and increase of calpain activity in the NSC-125066 treated mouse lung tissues.
    Molecular Weight

    362.46

    Formula

    C20H30N2O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OCC1=CC=CC=C1)N[C@H](C(N[C@H](C=O)CCCC)=O)CC(C)C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (275.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7589 mL 13.7946 mL 27.5893 mL
    5 mM 0.5518 mL 2.7589 mL 5.5179 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
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    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 5 mg/mL (13.79 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.17%

    SDS (396 KB)

    COA (245 KB) HNMR (290 KB) RP-HPLC (226 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7589 mL 13.7946 mL 27.5893 mL 68.9731 mL
    5 mM 0.5518 mL 2.7589 mL 5.5179 mL 13.7946 mL
    10 mM 0.2759 mL 1.3795 mL 2.7589 mL 6.8973 mL
    15 mM 0.1839 mL 0.9196 mL 1.8393 mL 4.5982 mL
    20 mM 0.1379 mL 0.6897 mL 1.3795 mL 3.4487 mL
    25 mM 0.1104 mL 0.5518 mL 1.1036 mL 2.7589 mL
    30 mM 0.0920 mL 0.4598 mL 0.9196 mL 2.2991 mL
    40 mM 0.0690 mL 0.3449 mL 0.6897 mL 1.7243 mL
    50 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3795 mL
    60 mM 0.0460 mL 0.2299 mL 0.4598 mL 1.1496 mL
    80 mM 0.0345 mL 0.1724 mL 0.3449 mL 0.8622 mL
    100 mM 0.0276 mL 0.1379 mL 0.2759 mL 0.6897 mL
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    Calpeptin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Calpeptin117591-20-5CathepsinProteasomeApoptosisInhibitorinhibitorinhibit

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