2. Academic Validation
  3. Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis

Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis

  • Biol Proced Online. 2025 Dec 22;27(1):48. doi: 10.1186/s12575-025-00299-w.
Ganhua You 1,  Chen Qian 2,  Xiaoyue Chen 3,  Qunying An 4,  Jukun Song 5,  Yuehai Xiao 6
Affiliations

Affiliations

  • 1 Department of Scientific Research, The Second People's Hospital of Guizhou Province, Guiyang, 550004, China.
  • 2 Department of Medical Quality Management, The 925th Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Guiyang, 550000, China.
  • 3 Department of Urology, The Affiliated Hospital of Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang, 550000, Guizhou Province, China.
  • 4 Department of General Medicine, The Sixth People's Hospital of Hengshui, Hengshui, 053200, China.
  • 5 Maxillofacial Surgery, The Stomatological Hospital of Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang, 550000, Guizhou Province, China. [email protected].
  • 6 Department of Urology, The Affiliated Hospital of Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang, 550000, Guizhou Province, China. [email protected].
PMID: 41430119 DOI: 10.1186/s12575-025-00299-w
Abstract

Background: Prostate Cancer (PCa) is the most common male reproductive malignancy, with traditional Chinese medicine (TCM) offering potential complementary treatments. Aidi injection, used for liver, lung, and colorectal cancers, shows promise for PCa, but its mechanisms are unclear. This study aims to uncover the molecular pathways through which Aidi injection affects PCa using network pharmacology and experiments.

Methods: The study used network pharmacology to identify active components of Aidi injection and their targets from various databases. These targets were cross-referenced with PCa-related targets to uncover therapeutic possibilities. Protein-protein interaction (PPI) and KEGG pathway analyses highlighted key molecular pathways affected by Aidi injection. Molecular docking examined ingredient-target binding. In vitro tests evaluated effects on PCa cell proliferation, Apoptosis, and IL-6/STAT3 signaling, while in vivo studies assessed tumor growth and immune response in mice.

Results: The study identified 58 active components and 804 PCa-related target genes, with 49 overlapping therapeutic targets. Key compounds such as quercetin, kaempferol, isorhamnetin, and cantharidin were highlighted. Core targets included ESR1, IL-6, STAT3, HSP90AA1, EGFR, and VEGFA. KEGG analysis revealed the IL-6/STAT3 and EGFR pathways as significant in PCa treatment. In vitro, Aidi injection inhibited PCa cell proliferation, induced Apoptosis, and suppressed IL-6/STAT3 activation. In vivo, Aidi injection reduced tumor growth and enhanced immune response in mice.

Conclusions: Aidi injection influences the IL-6/STAT3 and EGFR pathways, suppressing PCa cell growth, inducing Apoptosis, and reducing migration and invasion. These findings offer valuable insights into the therapeutic potential of Aidi injection for prostate Cancer.

Keywords

Aidi injection; Complementary medicine; IL-6/STAT3; Network pharmacology; Prostate cancer.

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