2. Academic Validation
  3. Quantification of Stress- and Resistance-Related Metabolites in Barley Leaves (Hordeum vulgare L.) Infected with Bipolaris sorokiniana via UHPLC-MS/MSMRM

Quantification of Stress- and Resistance-Related Metabolites in Barley Leaves (Hordeum vulgare L.) Infected with Bipolaris sorokiniana via UHPLC-MS/MSMRM

  • J Agric Food Chem. 2026 Jul 1;74(25):20051-20061. doi: 10.1021/acs.jafc.6c01645.
Lisa Kurzweil 1,  Timo D Stark 2,  Karina Hille 2,  Felix Hoheneder 3,  Jana Mrtva 2,  Hans Hausladen 4,  Miriam Lenk 5,  Mohammed Saddik Motawie 6,  Corina Vlot-Schuster 5 7,  Klaus Pillen 8,  Mette Sørensen 6 9,  Birger Lindberg Møller 6,  Ralph Hückelhoven 3,  Corinna Dawid 1 2 10 11
Affiliations

Affiliations

  • 1 Professorship for Functional Phytometabolomics, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, Freising 85354, Germany.
  • 2 Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, Freising 85354, Germany.
  • 3 Chair of Phytopathology, TUM School of Life Sciences, Technical University of Munich, Emil-Ramann-Str. 2, Freising 85354, Germany.
  • 4 Plant Technology Center, TUM School of Life Sciences, Technical University of Munich, Dürnast 9, Freising 85354, Germany.
  • 5 Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstraße 1, Neuherberg 85764, Germany.
  • 6 Plant Biochemistry Laboratory, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiskberg C, Copenhagen 1871, Denmark.
  • 7 Chair of Crop Plant Genetics, Faculty of Life Sciences: Food, Nutrition and Health, University of Bayreuth, Fritz-Hornschuch-Straße 13, Kulmbach 95326, Germany.
  • 8 Chair of Plant Breeding, Martin-Luther-University Halle-Wittenberg, Betty-Heimann-Str. 3, Halle (Saale) 06120, Germany.
  • 9 LEO Pharma, Industriparken 55, Ballerup, Copenhagen 5750, Denmark.
  • 10 Professorship for Chemosensory Food Systems, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, Freising 85354, Germany.
  • 11 Leibniz Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, Freising 85354, Germany.
PMID: 42299507 DOI: 10.1021/acs.jafc.6c01645
Abstract

This study investigated the quantitative changes in 33 stress- or resistance-related metabolites induced by Bipolaris sorokiniana in barley leaves of quantitatively resistant and susceptible barley lines of the multiparental nested association mapping (NAM) population HEB-25. The analyses were based on ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Twenty-nine infected and noninfected barley genotypes were analyzed at four different time points after inoculation. The method provided quantification of hordatines, phenolamides, hydroxycinnamic acids, flavone glucosides, hydroxynitrile glucosides, apocarotenoids, and indole derivatives. In leaves infected with B. sorokiniana, phenolamide levels were elevated compared to noninfected Plants. A correlation between metabolite levels and the severity of Infection showed that the more resistant barley lines contained higher amounts of hordatines and hordatine glucosides.

Keywords

Bipolaris sorokiniana; LC-MS/MS; barley; biotic stress; metabolites; quantification.

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