MitoTracker Orange CMTMRos

Name: MitoTracker Orange CMTMRos
Brand: MedChemExpress
SKU: HY-D1696
Rating: 4.5 (1 reviews)

製品番号: HY-D1696 純度: 94.19%: Data Sheet SDS COA 取扱説明書
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MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

MitoTracker Orange CMTMRos 構造式

CAS 番号 : 199116-50-2

容量	価格（税別）	在庫状況	数量
1 mg	$280	在庫あり	Estimated Time of Arrival: December 31
5 mg	$980	在庫あり	Estimated Time of Arrival: December 31
10 mg	$1650	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 1 publication(s) in Google Scholar

顧客検証

•Mol Plant. 2024 Dec 2;17(12):1899-1918. [Abstract]

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生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm)^[1].

IC₅₀ & Target

Mitochondria^[1]

体外実験

Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Cell labeling^[1]:
For live cells, follow steps 1-7. For fixed cells, follow steps 8-13.
1. Prepare a labeling solution at a concentration of 25–500 nM in the desired cell culture medium, and warm it to the cell culture temperature.
2. Remove the culture medium from the cells on the coverslip.
3. Immerse the cells in the labeling medium and incubate at 37°C for 15-45 min.
4. Remove the labeling medium and rinse the cells three times with culture medium.
5. Mount the cells.
6. Examine the cells using a fluorescence microscope. If fluorescence staining is too low, try one of the following:
i. Incubate for an additional 30 min in normal culture medium to allow thiol binding to occur.
ii. Increase the initial concentration of the labeling solution.
7. When fluorescence is sufficient, image the cells.
8. Repeat steps 1-4, but use a labeling solution with a working concentration between 100 and 1000 nM.
9. Wash the cells three times with PBS.
10. Fix the cells in PBS (pH 7.4) containing 3.7% formaldehyde for 10 min.
11. Aspirate and wash the cells three times with PBS, 5 min each.
12. Mount with a coverslip.
13. Image the cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

427.37

分子式

C₂₄H₂₄Cl₂N₂O

Emission (Em)

576

Excitation (Ex)

554

CAS 番号

199116-50-2

Appearance

Solid

Color

Brown to black

SMILES

CN(C)C(C=C1)=CC2=C1C(C3=CC=C(CCl)C=C3)=C(C=C/4)C(O2)=CC4=[N+](C)/C.[Cl-]

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 31.25 mg/mL (73.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3399 mL	11.6995 mL	23.3989 mL
5 mM	0.4680 mL	2.3399 mL	4.6798 mL
10 mM	0.2340 mL	1.1699 mL	2.3399 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 94.19%

Select Batch:

データシート (275 KB) SDS (252 KB)

COA (250 KB) HNMR (179 KB) RP-HPLC (232 KB) MS (69 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Chazotte B. Labeling mitochondria with MitoTracker dyes. Cold Spring Harb Protoc. 2011 Aug 1;2011(8):990-2. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3399 mL	11.6995 mL	23.3989 mL	58.4973 mL
	5 mM	0.4680 mL	2.3399 mL	4.6798 mL	11.6995 mL
	10 mM	0.2340 mL	1.1699 mL	2.3399 mL	5.8497 mL
	15 mM	0.1560 mL	0.7800 mL	1.5599 mL	3.8998 mL
	20 mM	0.1170 mL	0.5850 mL	1.1699 mL	2.9249 mL
	25 mM	0.0936 mL	0.4680 mL	0.9360 mL	2.3399 mL
	30 mM	0.0780 mL	0.3900 mL	0.7800 mL	1.9499 mL
	40 mM	0.0585 mL	0.2925 mL	0.5850 mL	1.4624 mL
	50 mM	0.0468 mL	0.2340 mL	0.4680 mL	1.1699 mL
	60 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9750 mL