MK-28

Name: MK-28
Brand: MedChemExpress
SKU: HY-137207
Rating: 5.0 (9 reviews)

Cat. No.: HY-137207 Purity: 99.88%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

MK-28 is a potent and selective PERK activator. MK-28 exhibits remarkable pharmacokinetic properties and high BBB penetration in mice.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 864388-65-8

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	견적 받기
10 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 9 publication(s) in Google Scholar

9 Publications Citing Use of MCE MK-28

RT-PCR

Histological Imaging/Staining

Bio/Physico-chemical Assay

: MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]; Representative images of UCP1 immunofluorescence staining. SVF cells isolated from iWAT of 2-month-old male mice were pre-treated with either vehicle, E2 (10 nM), or three different activators of ER stress MK-28, IXA4, AA147 (10 μM) before differentiating into beige adipocytes.

: MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]; Western blot analysis of PERK and PERK phosphorylation in iWAT from vehicle and MK-28 (10 μM).

: MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]; qPCR analysis of thermogenic gene expression treated with MK-28 (10 μM).

: MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]; Representative images of H&E staining in the middle part of iWAT treated with MK-28 (1 mg/kg, i.p.).

: MK-28 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Jun;4(6):839-853. [Abstract]; The blood glucose levels of the vehicle or MK28 (1 mg/kg, i.p.) treated group in 2-month-old male mice after intraperitoneal injection of glucose tolerance test and Area under curve (AUC) analysis by one way-ANOVA followed by Tukey’s post-hoc test.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

MK-28 is a potent and selective PERK activator. MK-28 exhibits remarkable pharmacokinetic properties and high BBB penetration in mice^[1].

In Vitro

MK-28 (0-100 μM) shows PERK selectivity in vitro on a 391-kinase panel and rescues cells (but not PERK^/ cells) from ER stress-induced apoptosis^[1].
ATF4 protein levels are increased signifcantly, up to 2.5-fold, in STHdhQ^111/111 cells at high MK-28 concentration. CHOP and GADD34 mRNA levels show a signifcant increase in both cell types, up to 10- and 5-fold respectively^[1].
MK-28 has little or no efect on EIF2AK1 (HRI) or EIF2AK2 (PKR), but it activats EIF2AK4 (GCN2)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1].

Cell Line:	STHdhQ^111/111 cells.
Concentration:	0-100 μM.
Incubation Time:	48 h.
Result:	Rescued cells from ER stress-induced apoptosis.

In Vivo

MK-28 (10 mg/kg, IP, single dose) shows a maximum concentration (C_max) of 105ng/ml and 30min half-life in plasma, 40 min afer the IP injection^[1].
MK-28 (1 mg/kg, IP, daily for 28 days) improves systemic function and survival in R6/2 mice and induces increased levels of eIF2α-P in the mouse brain striatum^[1].
MK-28 (Transient subcutaneous delivery) significantly improves motor and executive functions and delayed death onset in R6/2 mice, showing no toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	R6/2 mice^[1].
Dosage:	1 mg/kg.
Administration:	IP, daily for 28 days.
Result:	Incerased the survival.

분자량

396.44

화학식

C₂₄H₂₀N₄O₂

CAS No.

864388-65-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=CC=C(C=C1O)/C=N/N(C2=NC(C3=CC=CC=C3)=CC(C4=CC=CC=C4)=N2)C

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 12.5 mg/mL (31.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5224 mL	12.6122 mL	25.2245 mL
5 mM	0.5045 mL	2.5224 mL	5.0449 mL
10 mM	0.2522 mL	1.2612 mL	2.5224 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.25 mg/mL (3.15 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.88%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (248 KB) HNMR (231 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Javier Ganz, et al. A novel specific PERK activator reduces toxicity and extends survival in Huntington's disease models. Sci Rep. 2020 Apr 23;10(1):6875. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5224 mL	12.6122 mL	25.2245 mL	63.0612 mL
	5 mM	0.5045 mL	2.5224 mL	5.0449 mL	12.6122 mL
	10 mM	0.2522 mL	1.2612 mL	2.5224 mL	6.3061 mL
	15 mM	0.1682 mL	0.8408 mL	1.6816 mL	4.2041 mL
	20 mM	0.1261 mL	0.6306 mL	1.2612 mL	3.1531 mL
	25 mM	0.1009 mL	0.5045 mL	1.0090 mL	2.5224 mL
	30 mM	0.0841 mL	0.4204 mL	0.8408 mL	2.1020 mL

MK-28 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MK-28864388-65-8MK28MK 28PERKProtein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinaseHuntingtonER-stressInhibitorinhibitorinhibit

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MK-28

고객리뷰

MK-28 Related Antibodies

9 Publications Citing Use of MCE MK-28

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

MK-28 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

MK-28 Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report