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NIR-H2O2 

Cat. No.: HY-D1065
Handling Instructions

NIR-H2O2 is a cell-permeable near-infrared (NIR) fluorescent turn-on sensor. NIR-H2O2 has both absorption and emission in the NIR region. NIR-H2O2 responds to H2O2 with a large turn-on NIR fluorescence signal upon excitation in the NIR region. NIR-H2O2 is capable of imaging endogenously produced H2O2 in living cells and mice.

For research use only. We do not sell to patients.

NIR-H2O2 Chemical Structure

NIR-H2O2 Chemical Structure

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Description

NIR-H2O2 is a cell-permeable near-infrared (NIR) fluorescent turn-on sensor. NIR-H2O2 has both absorption and emission in the NIR region. NIR-H2O2 responds to H2O2 with a large turn-on NIR fluorescence signal upon excitation in the NIR region. NIR-H2O2 is capable of imaging endogenously produced H2O2 in living cells and mice[1].

In Vitro

NIR-H2O2 is highly selective to H2O2 over other typical ROS and biorelevant species[1].
HeLa cells incubated with NIR-H2O2 (5 μM) for 30 min at 37 °C provide almost no fluorescence. However, when the living HeLa cells loaded with NIR-H2O2 are further treated with H2O2, they give strong fluorescence. NIR-H2O2 is cell membrane permeable and responsive to H2O2 in the living cells. When stimulated by phorbol myristate acetate (PMA), macrophage cells may produce endogenous H2O2. The living RAW264.7 macrophage cells loaded with only the NIR sensor NIR-H2O2 (1 μM) display almost no fluorescence. However, the macrophage cells coincubated with PMA (3.0 μg/mL) and the sensor NIR-H2O2 (1 μM) exhibit a dramatic enhancement in the red emission. NIR-H2O2 is capable of fluorescent imaging of endogenously produced H2O2 in the living RAW264.7 macrophage cells. The mitochondria staining experiments suggest that the sensor mainly associates with the mitochondria of RAW264.7 macrophage cells[1].

In Vivo

The H2O2 production in vivo was generated by activated macrophages and neutrophils in a lipopolysaccharide (LPS) model of acute inflammation. The mice treated with both LPS and NIR-H2O2 exhibit a significantly higher fluorescence readout than the mice untreated or treated with only NIR-H2O2. The mice loaded with LPS and NIR-H2O2 have approximately 10- and 20-fold higher fluorescence intensity than the mice loaded with saline and the sensor and the mice loaded with saline, respectively[1].

Molecular Weight

565.89

Formula

C₃₄H₃₃BClNO₄

SMILES

ClC1=CC2=C(OC3=C(CCCC3=C2)/C=C/C4=[N+](C5=C(C4(C)C)C=CC=C5)CC)C=C1OCC6=CC=C(C=C6)B([O-])O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

NIR-H2O2OthersNIRfluorescentsensorregionendogenouslyH2O2living-cellsliving-miceturn-onInhibitorinhibitorinhibit

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