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  4. 4-Nitrophenyl phosphate disodium

PNPP disodium  (Synonyms: Para-nitrophenyl phosphate disodium)

製品番号: HY-15928 純度: 99.85%
COA 取扱説明書 Technical Support

4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

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4-Nitrophenyl phosphate disodium

PNPP disodium 構造式

CAS 番号 : 4264-83-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in Water USD 33 在庫あり
Solid
500 mg $30 在庫あり
1 g $45 在庫あり
5 g $96 在庫あり
10 g   お問い合わせ  
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

Other Forms of 4-Nitrophenyl phosphate disodium:

PNPP disodium 関連抗体

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製品説明

4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

体外実験

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PNPP disodium 関連抗体
分子量

263.05

分子式

C6H4NNa2O6P
CAS 番号
Appearance

Solid

Color

White to yellow

SMILES

O=P(O[Na])(O[Na])OC1=CC=C([N+]([O-])=O)C=C1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

H2O : 160 mg/mL (608.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8016 mL 19.0078 mL 38.0156 mL
5 mM 0.7603 mL 3.8016 mL 7.6031 mL
10 mM 0.3802 mL 1.9008 mL 3.8016 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.85%

COA (254 KB) HNMR (154 KB) LCMS (348 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.8016 mL 19.0078 mL 38.0156 mL 95.0390 mL
5 mM 0.7603 mL 3.8016 mL 7.6031 mL 19.0078 mL
10 mM 0.3802 mL 1.9008 mL 3.8016 mL 9.5039 mL
15 mM 0.2534 mL 1.2672 mL 2.5344 mL 6.3359 mL
20 mM 0.1901 mL 0.9504 mL 1.9008 mL 4.7519 mL
25 mM 0.1521 mL 0.7603 mL 1.5206 mL 3.8016 mL
30 mM 0.1267 mL 0.6336 mL 1.2672 mL 3.1680 mL
40 mM 0.0950 mL 0.4752 mL 0.9504 mL 2.3760 mL
50 mM 0.0760 mL 0.3802 mL 0.7603 mL 1.9008 mL
60 mM 0.0634 mL 0.3168 mL 0.6336 mL 1.5840 mL
80 mM 0.0475 mL 0.2376 mL 0.4752 mL 1.1880 mL
100 mM 0.0380 mL 0.1901 mL 0.3802 mL 0.9504 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Nitrophenyl phosphate disodium Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl phosphate disodium4264-83-9p-Nitrophenyl phosphate disodiumFluorescent DyeInhibitorinhibitorinhibit

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製品名:
4-Nitrophenyl phosphate disodium
製品番号:
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