Recombinant DNase I (RNase-free)

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Recombinant DNase I (RNase-free) is a recombinant deoxyribonuclease that degrades DNA. Recombinant DNase I is essential for limiting inflammatory responses and maintaining homeostasis.

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CAS No. : 9003-98-9

Size	Stock	Quantité
200 U	En stock	Estimated Time of Arrival: December 31
500 U	En stock	Estimated Time of Arrival: December 31
1 KU	En stock	Estimated Time of Arrival: December 31

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Based on 29 publication(s) in Google Scholar

Other Forms of Recombinant DNase I (RNase-free):

DNase I, Bovine pancreas In-stock
DNase I RNase & Protease free In-stock

29 Publications Citing Use of MCE Recombinant DNase I (RNase-free)

Histological Imaging/Staining

Cell Imaging/Staining

Cell Migration/Invasion Assay

: Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]; Masson staining was conducted to detect fibrosis in tissue samples from different groups. Sham: 3.29% ± 0.64%, MIRI: 30.28% ± 5.87%, MIRI + DNase I: 15.91% ±2.92%, MIRI + GSK484: 17.12% ± 2.48%.

: Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 May 30:8:0705. [Abstract]; IF was performed to detect the expression of VE-cadherin of HUVECs under different treatments. Neutrophils/Sham: 136.08 ± 20.98, Neutrophils/MIRI: 69.45 ± 8.91, Neutrophils/MIRI + DNase I: 108.14 ± 12.38, Neutrophils/MIRI + GSK484: 111.44 ± 16.16.

: Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Sep 5;10(1):395. [Abstract]; The whole aorta was detected by western blot, and the expressionof NETs products (ELANE, MPO) was quantitatively analyzed treatd with DNase I (4 mg/kg, intraperitoneally daily for 3 weeks).

: Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]; The effect of NETs on the proliferation of Cal27 cells was detected by EdU proliferation assay DNase I (1 μg/mL,37°C, 15 min).

: Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]; The effect of NETs on the invasion of Cal27 cells was detected by Transwell invasion assay DNase I (1 μg/mL,37°C, 15 min).

: Recombinant DNase I (RNase-free) purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 May 2;10(1):214. [Abstract]; The effect of NETs on the migration of Cal27 cells was detected by wound healing assay treated with DNase I (1 μg/mL,37°C, 15 min).

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Description	Recombinant DNase I (RNase-free) is a recombinant deoxyribonuclease that degrades DNA. Recombinant DNase I is essential for limiting inflammatory responses and maintaining homeostasis^[1].
In Vitro	Product Information DNase I is a non-specific endonuclease that degrades double-stranded and single-stranded DNA as well as chromosomes. It functions by hydrolyzing phosphodiester bonds to generate mononucleotides and oligonucleotides with 5'-phosphate and 3'-hydroxyl groups. The enzyme activity requires the presence of Mg²⁺ or Ca²⁺ ions, with an optimal pH of approximately 7.8. This product is recombinant DNase I derived from bovine pancreas and expressed in Pichia pastoris, with RNase activity removed during purification. The storage buffer consists of 10 mM Tris, 2 mM CaCl₂, 50% glycerol, pH 7.6 Removal of DNA Contamination from RNA 1. The reaction system contains: 10 μg RNA, 10 μL of 10× DNase I Reaction Buffer (100 mM Tris, 5 mM CaCl₂, 25 mM MgCl₂, pH 7.6), 2 U of recombinant DNase I, and water to make up the final volume to 100 μL. 2. Incubate at 37 ℃ for 10 min. After the reaction is complete, add EDTA to a final concentration of 5 mM and incubate at 75 ℃ for 10 min to inactivate DNase I. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Recombinant DNase I (RNase-free) Related Antibodies
CAS No.	9003-98-9
Appearance	Liquid
Color	Colorless to light yellow
SMILES	[Recombinant DNase I (RNase-free)]
EC Number	3.1.21.1
Enzyme Activity	≥ 2 U/μL
Unit Definition	One unit is defined as the amount of enzyme required to completely degrade 1 μg of plasmid DNA in 10min at 37°C
Livraison	Room temperature in continental US; may vary elsewhere.
Stockage	Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation	Select Batch: Fiche technique (268 KB) SDS (419 KB) COA (243 KB) Instruction de manipulation (2659 KB)
Références	[1]. Lauková L, et al. Deoxyribonucleases and Their Applications in Biomedicine. Biomolecules. 2020 Jul 11;10(7):1036. [Content Brief] [2]. Sugihara S, et al. Deoxyribonuclease treatment prevents blood-borne liver metastasis of cutaneously transplanted tumour cells in mice. Br J Cancer. 1993 Jan;67(1):66-70. [Content Brief]