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  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
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  4. IL-26
  5. Animal-Free IL-26/AK155 Protein, Human (His)

Animal-Free IL-26/AK155 Protein, Human (His)

Cat. No.: HY-P700117AF
COA Handling Instructions

The IL-26/AK155 Monomer protein potentially plays a crucial role in mucosal immunity, with a pro-inflammatory function linked to inflammatory bowel disease. Functionally, it activates various signaling pathways, induces the expression of immune mediators, and decreases intestinal epithelial cell proliferation. Its oligomeric state, forming homodimers, emphasizes its significance in mediating cellular responses and signaling cascades within mucosal immunity. Animal-Free IL-26/AK155 Protein, Human (His) is the recombinant human-derived animal-FreeIL-26/AK155 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free IL-26/AK155 Protein, Human (His) is 150 a.a., with molecular weight of ~18.53 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Description

The IL-26/AK155 Monomer protein potentially plays a crucial role in mucosal immunity, with a pro-inflammatory function linked to inflammatory bowel disease. Functionally, it activates various signaling pathways, induces the expression of immune mediators, and decreases intestinal epithelial cell proliferation. Its oligomeric state, forming homodimers, emphasizes its significance in mediating cellular responses and signaling cascades within mucosal immunity. Animal-Free IL-26/AK155 Protein, Human (His) is the recombinant human-derived animal-FreeIL-26/AK155 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free IL-26/AK155 Protein, Human (His) is 150 a.a., with molecular weight of ~18.53 kDa.

Background

The IL-26/AK155 Monomer protein is implicated in potentially playing a crucial role in local mechanisms of mucosal immunity, suggesting its involvement in the intricate processes of immune regulation within mucosal tissues. It appears to have a pro-inflammatory function and may be associated with inflammatory bowel disease, underscoring its significance in inflammatory processes in the gastrointestinal tract. Functionally, IL-26/AK155 Monomer activates various signaling pathways, including STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN, and AKT, suggesting a broad impact on cellular responses. It induces the expression of SOCS3, TNF-alpha, and IL-8, along with the secretion of IL-8 and IL-10, as well as the surface expression of ICAM1, highlighting its involvement in the regulation of immune and inflammatory mediators. Additionally, IL-26/AK155 Monomer demonstrates the ability to decrease the proliferation of intestinal epithelial cells. Notably, its activity is inhibited by heparin, adding a layer of regulatory control. The structural arrangement involves the formation of homodimers, emphasizing the importance of its oligomeric state in mediating cellular responses and signaling cascades within the context of mucosal immunity.

Biological Activity

Measure by its ability to induce IL-10 secretion in COLO205 cells. The ED50 for this effect is <150 ng/mL

Species

Human

Source

E. coli

Tag

C-His

Accession

Q9NPH9 (K22-Q171)

Gene ID
Molecular Construction
N-term
IL-26 (K22-Q171)
Accession # Q9NPH9
His
C-term
Synonyms
AK155; IL-26; Interleukin-26; Protein AK155; Interleukin 26
AA Sequence

MKHKQSSFTKSCYPRGTLSQAVDALYIKAAWLKATIPEDRIKNIRLLKKKTKKQFMKNCQFQEQLLSFFMEDVFGQLQLQGCKKIRFVEDFHSLRQKLSHCISCASSAREMKSITRMKRIFYRIGNKGIYKAISELDILLSWIKKLLESSQ

Molecular Weight

Approximately 18.53 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 8.0.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Animal-Free IL-26/AK155 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free IL-26/AK155 Protein, Human (His)
Cat. No.:
HY-P700117AF
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