AXL Protein, Human (Biotinylated, HEK293, Fc)

AXL protein is a receptor tyrosine kinase that mediates signals by binding to GAS6 and regulates cell survival, proliferation, migration and differentiation. After ligand binding, AXL dimerizes and autophosphorylates, activating downstream molecules, such as PI3 kinase subunits, GRB2, PLCG1, etc. AXL Protein, Human (Biotinylated, HEK293, Fc) is the recombinant human-derived AXL protein, expressed by HEK293 , with C-hFc labeled tag.

The AXL protein, a receptor tyrosine kinase, serves as a key mediator in transducing signals from the extracellular matrix into the cytoplasm by binding the growth factor GAS6, thereby regulating diverse physiological processes such as cell survival, proliferation, migration, and differentiation. Ligand binding at the cell surface induces AXL dimerization and autophosphorylation. Upon activation, AXL interacts with and induces the tyrosine phosphorylation of various downstream signaling molecules, including PI3-kinase subunits (PIK3R1, PIK3R2, and PIK3R3), GRB2, PLCG1, LCK, PTPN11, CBL, NCK2, SOCS1, and TNS2. This triggers the recruitment of GRB2 and regulatory subunits of phosphatidylinositol 3 kinase, leading to the downstream activation of the AKT kinase. The GAS6/AXL signaling axis plays a pivotal role in various processes such as endothelial cell survival, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, and the regulation of thrombotic responses. Additionally, AXL is involved in inhibiting Toll-like receptors (TLRs)-mediated innate immune responses, and in the context of microbial infection, it acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of the virion envelope.

1.This product does not contain protein kinase domain.
2.Immobilized Anti-Axl Antibody at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC₅₀ of ≤30 ng/mL determined by ELISA.
3.Immobilized Human GAS6, His Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC₅₀ of ≤12 ng/mL determined by ELISA.

Human

HEK293

C-hFc

P30530-1 (A26-P449)

558  [NCBI]

Extracellular Domain

Biotin

APRGTQAEESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWP

80-110 kDa

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.