Cadherin-1/CD324 Protein, Rat (HEK293, Fc)

Cadherin-1/CD324 protein, a calcium-dependent adhesion molecule, mediates cell processes. It interacts homophilically with other cadherin molecules, sorting and organizing cell types. CDH1 regulates adhesion, mobility, and proliferation of epithelial cells. It serves as an integrin ligand, suppressing invasion and promoting non-amyloidogenic degradation. CDH1 inhibits APP C99 and C83 production. Cadherin-1/CD324 Protein, Rat (HEK293, Fc) is the recombinant rat-derived Cadherin-1/CD324 protein, expressed by HEK293, with C-hFc labeled tag. The total length of Cadherin-1/CD324 Protein, Rat (HEK293, Fc) is 690 a.a., with molecular weight of 95-130 kDa.

Cadherin-1/CD324 protein, a calcium-dependent cell adhesion molecule, plays a pivotal role in various cellular processes. By preferentially interacting with other cadherin molecules of the same type in a homophilic manner, CDH1 contributes to the sorting and organization of different cell types within tissues. Furthermore, it is involved in regulating cell-cell adhesions, mobility, and proliferation of epithelial cells. CDH1 also serves as a ligand for integrin alpha-E/beta-7 and exhibits a potent suppressor role in invasion. Additionally, it promotes the non-amyloidogenic degradation of Abeta precursors and exerts a strong inhibitory effect on the production of APP C99 and C83.

Measured by the ability of the immobilized protein to support the adhesion of P19 mouse embryonal carcinoma cells. The ED₅₀ for this effect is 0.0915 μg/mL. Corresponding to a specific activity is 1.093×10⁴ U/mg.

Rat

HEK293

C-hFc

Q9R0T4 (Q24-A713)

83502  [NCBI]

QPESESDSCRPGFSSEVYTFLVPERHLERGHILGRVKFEGCTGRPRTAFFSEDSRFKVSTDGVITVKRHLKLHKLETSFLVHAWDSSYRKLSTKVTLKSLGHHHHRHHHRDPVSESNPELLTFPSFHQGLRRQKRDWVIPPINCPENQKGEFPQRLVQIKSNRDKETTVFYSITGPGADKPPVGVFIIERETGWLKVTQPLDREAIDKYLLYSHAVSSNGEAVEDPMEIVVTVTDQNDNRPEFIQEVFEGSVAEGALPGTSVMQVSATDADDDINTYNAAIAYTILSQDPELPHKNMFTVNRDTGVISVVTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPIFNPSTYQGQVLENEVGARIATLKVTDDDAPNTPAWNAVYTVVNDPDHQFTVITDPKTNEGILKTAKGLDFEAKQQYILHVTVENEEPFEGSLVPSTATVTVDVVDVNEAPIFVPAEKRVEVPEDFGVGLEIASYTAREPDTFMEQKITYRIWRDTANWLEINPETGVISTRAEMDREDSEHVKNSTYTALIIATDDGSPIATGTGTLLLVLSDVNDNAPIPEPRNMQFCQRNPKPHVITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAEQESLILQPRKDLEIGEYKINLKLSDNQNKDQVTTLEVHVCDCEGTVNNCMKAISLEAGLQVPA

95-130 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.