1. Recombinant Proteins
  2. CTLA-4 Protein, Human (HEK293, GST)

CTLA-4 Protein, Human (HEK293, GST)

Cat. No.: HY-P70691
Handling Instructions

CTLA-4 Protein, Human (HEK293, GST) is negative regulator of T-cell immune function.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $80 In-stock
Estimated Time of Arrival: December 31
50 μg $240 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4
Species

Human

Source

HEK 293

Accession

P16410 (K36-D161)

Gene ID

1493

Molecular Weight

40-55 kDa

AA Sequence

KAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSD

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity
5 μg/mL (100 μL/well) of immobilized Mouse B7-1-Fc can bind Biotinylated Human CTLA-4-GST with an ED50 value of 1.17 μg/mL.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Background

CTLA-4 is the member of a family of Immunoglobulin-related receptors that are responsible for various aspects of T cell immune regulation. The family includes CD28, CTLA-4 and ICOS as well as other proteins including PD-1, BTLA and TIGIT. CTLA-4 is predominantly found in intracellular vesicles in FoxP3+ Treg cells or activated conventional T cells. CTLA-4 can compete with CD28 for ligand binding and thereby act as an antagonist of CD28-mediated co-stimulation[1][2][3].

Documentation
References
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Product Name:
CTLA-4 Protein, Human (HEK293, GST)
Cat. No.:
HY-P70691
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