CYB5R1 Protein, Human (His)

CYB5R1 is an important member of the NADH-cytochrome b5 reductase family and plays a crucial role in fatty acid desaturation, cholesterol biosynthesis, drug metabolism, and erythrocyte methemoglobin reduction. Utilizing NADH, CYB5R1 promotes the reduction of cytochrome b5, contributing to enzymatic processes in lipid metabolism, sterol synthesis, and drug biotransformation. CYB5R1 Protein, Human (His) is the recombinant human-derived CYB5R1 protein, expressed by E. coli , with N-His labeled tag.

CYB5R1, a member of the NADH-cytochrome b5 reductase family, plays a crucial role in diverse biological processes such as fatty acid desaturation and elongation, cholesterol biosynthesis, drug metabolism, and methemoglobin reduction in erythrocytes. As an essential component of these metabolic pathways, CYB5R1 utilizes NADH as a cofactor to facilitate the reduction of cytochrome b5, contributing to the enzymatic processes involved in lipid metabolism, sterol synthesis, and drug biotransformation. In erythrocytes, CYB5R1's involvement in methemoglobin reduction underscores its significance in maintaining hemoglobin functionality and preventing oxidative stress. The multifunctional nature of CYB5R1 positions it as a key player in cellular processes crucial for lipid homeostasis, hemoglobin function, and drug metabolism.

Measured by its ability to combine with the NADPH produces the H₂O₂. The specific activity is 7661.210 pmoL/min/μg, as measured under the described conditions.

Human

E. coli

N-His

Q9UHQ9 (L29-Y305)

51706  [NCBI]

Partial

LVRRSRRPQVTLLDPNEKYLLRLLDKTTVSHNTKRFRFALPTAHHTLGLPVGKHIYLSTRIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKVGDVVEFRGPSGLLTYTGKGHFNIQPNKKSPPEPRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTEKDIILREDLEELQARYPNRFKLWFTLDHPPKDWAYSKGFVTADMIREHLPAPGDDVLVLLCGPPPMVQLACHPNLDKLGYSQKMRFTY

Approximately 34 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.