1. Recombinant Proteins
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphA4
  6. EphA4 Protein, Human (HEK293, His-Fc)

EphA4 Protein, Human (HEK293, His-Fc)

Cat. No.: HY-P72994
Handling Instructions

The EphA4 protein is a receptor tyrosine kinase that participates in bidirectional signaling with membrane-bound ephrin ligands including EFNA1 and EFNB3. EphA4 is activated by GPI-anchored ephrin A and transmembrane ephrin B and regulates cell morphology and integrin-dependent adhesion through regulation of Rac, Rap, and Rho GTPases. EphA4 Protein, Human (HEK293, His-Fc) is the recombinant human-derived EphA4 protein, expressed by HEK293 , with C-hFc, C-His labeled tag. The total length of EphA4 Protein, Human (HEK293, His-Fc) is 547 a.a., with molecular weight of 100-110 kDa.

For research use only. We do not sell to patients.

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Description

The EphA4 protein is a receptor tyrosine kinase that participates in bidirectional signaling with membrane-bound ephrin ligands including EFNA1 and EFNB3. EphA4 is activated by GPI-anchored ephrin A and transmembrane ephrin B and regulates cell morphology and integrin-dependent adhesion through regulation of Rac, Rap, and Rho GTPases. EphA4 Protein, Human (HEK293, His-Fc) is the recombinant human-derived EphA4 protein, expressed by HEK293 , with C-hFc, C-His labeled tag. The total length of EphA4 Protein, Human (HEK293, His-Fc) is 547 a.a., with molecular weight of 100-110 kDa.

Background

The EphA4 protein is a receptor tyrosine kinase that interacts with membrane-bound ephrin ligands on adjacent cells, enabling bidirectional signaling. It can be activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands, such as EFNA1 and EFNB3. Activation of EphA4 modulates cell morphology and integrin-dependent cell adhesion by regulating the activity of Rac, Rap, and Rho GTPases. In the nervous system, EphA4 plays a crucial role in axonal guidance, including corticospinal projections and segregation of motor and sensory axons during neuromuscular circuit development. It also contributes to synaptic plasticity and is involved in repair after injury, preventing axonal regeneration. EphA4's promiscuity allows it to participate in various cell-cell signaling processes, including the development of the thymic epithelium. Furthermore, it regulates pillar cell separation in the cochlear organ of Corti by forming a complex with ADAM10 and CADH1, leading to the disruption of adherens junctions. EphA4 phosphorylates CAPRIN1, promoting the formation of a membraneless compartment.

Species

Human

Source

HEK293

Tag

C-hFc;C-His

Accession

P54764 (M1-T547)

Gene ID
Molecular Construction
N-term
EphA4 (M1-T547)
Accession # P54764
hFc-His
C-term
Synonyms
Ephrin type-A receptor 4; EPH-like kinase 8; EK8; EPHA4; HEK8; SEK; TYRO1
AA Sequence

MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANST

Molecular Weight

100-110 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
EphA4 Protein, Human (HEK293, His-Fc)
Cat. No.:
HY-P72994
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