FGFR-4 Protein, Rat (HEK293, Fc, Solution)

The FGFR-4 protein is a tyrosine-protein kinase receptor for fibroblast growth factor that regulates cellular processes, lipid metabolism, bile acid biosynthesis, and more.Its effects include downregulation of CYP7A1 in response to FGF19.FGFR-4 Protein, Rat (HEK293, Fc, Solution) is the recombinant rat-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag.

FGFR-4 protein, a tyrosine-protein kinase, functions as a cell-surface receptor for fibroblast growth factors, playing a crucial role in the regulation of cell proliferation, differentiation, and migration. Additionally, it contributes to the control of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate homeostasis. Its significance is highlighted in the normal down-regulation of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Upon ligand binding, FGFR-4 activates various signaling cascades, including the phosphorylation of PLCG1 and FRS2. This activation leads to the production of cellular signaling molecules like diacylglycerol and inositol 1,4,5-trisphosphate. Furthermore, FRS2 phosphorylation triggers the recruitment of GRB2, GAB1, PIK3R1, and SOS1, activating RAS, MAPK1/ERK2, MAPK3/ERK1, the MAP kinase signaling pathway, and the AKT1 signaling pathway. Notably, FGFR-4 promotes SRC-dependent phosphorylation of the matrix protease MMP14, facilitating its lysosomal degradation. To regulate FGFR-4 signaling, the receptor undergoes internalization and degradation, a process facilitated by MMP14.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA.
3.Immobilized human FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4 , The EC₅₀ of Rat FGFR4 is 1.17 μg/mL.
4.Immobilized mouse FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4 , The EC₅₀ of Rat FGFR4 is 0.44 μg/mL.
5.Immobilized human bFGF at 10 μg/mL (100 μl/well) can bind Rat FGFR4 , The EC₅₀ of Rat FGFR4 is 0.163 μg/mL.

Rat

HEK293

C-hFc

Q498D6 (F17-D367)

25114  [NCBI]

Extracellular Domain

MWLLLALLSIFQETPAFSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAEEEDLAWTTATSEARYTD

Approximately 110 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.