GAPDH Protein, Human (His-SUMO)

GAPDH, a multifunctional protein, acts as a key enzyme in glycolysis, catalyzing the conversion of G3P. It also functions as a nitrosylase, impacting nuclear processes. In addition, it influences cytoskeleton organization, associates with CHP1, participates in transcript-selective translation inhibition, contributes to immune responses, and mediates nuclear events through S-nitrosylation of target proteins. GAPDH Protein, Human (His-SUMO) is the recombinant human-derived GAPDH protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) exhibits dual functionality, serving as both a key enzyme in glycolysis, catalyzing the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate, and as a nitrosylase with roles in nuclear functions. In glycolysis, GAPDH plays a pivotal role in the initial step of the pathway. Beyond its metabolic functions, GAPDH modulates the organization and assembly of the cytoskeleton, facilitating microtubule and membrane associations through interaction with CHP1. Additionally, it is a component of the GAIT complex, participating in interferon-gamma-induced transcript-selective translation inhibition during inflammatory processes. In the realm of innate immunity, GAPDH contributes to TNF-induced NF-kappa-B activation and type I interferon production through interactions with TRAF2 and TRAF3, respectively. Furthermore, GAPDH is involved in various nuclear events, including transcription, RNA transport, DNA replication, and apoptosis, with its nitrosylase activity mediating cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and PRKDC.

Specific activity is 20223 U/mg that incubate at 37ºC for 5 min. The consumption of 1 nmol of NADH per minute per mg of protein is defined as one enzyme activity unit.

Human

E. coli

N-6*His;N-SUMO

P04406-1 (G2-E335)

2597  [NCBI]

Full Length of Isoform-1 Mature Protein

GKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Approximately 55 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.