GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, Fc)

GFRA1/GDNFR-α-1 protein is a receptor for GDNF and critically mediates GDNF-induced autophosphorylation and RET receptor activation. Presumably, two GDNFR-α-1 molecules form a complex with disulfide-linked GDNF, two RET molecules, and SORL1, suggesting the existence of a coordinated signaling mechanism. GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, Fc) is 445 a.a., with molecular weight of ~85 kDa.

GFRA1/GDNFR-alpha-1 Protein serves as a receptor for GDNF and plays a crucial role in mediating the GDNF-induced autophosphorylation and activation of the RET receptor. It is proposed that two molecules of GDNFR-alpha-1 form a complex with the disulfide-linked GDNF dimer and two molecules of RET, suggesting a coordinated mechanism for signal transduction. GFRA1 interacts with RET, facilitating the intricate signaling pathways associated with GDNF-induced responses. Additionally, GFRA1 engages in interactions with SORL1, either alone or in a complex with GDNF. This interaction results in the internalization of GFRA1, without leading to its degradation, indicating a regulatory aspect of GFRA1 dynamics. These molecular interactions highlight the multifaceted role of GFRA1 in mediating GDNF signaling and emphasize its importance in cellular processes.

Immobilized rat His-GDNF (78-211) at 10 μg/mL (100 μl/well) can bind rat GFRA1-Fc, The EC₅₀ of rat GFRA1-Fc is 20-46.6 ng/mL.

Rat

HEK293

C-hFc

Q62997 (M1-L445)

25454  [NCBI]

MFLATLYFALPLLDLLMSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISDVFQQVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEERERPNCLSLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYVDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDSDFGKDGLAGASSHITTKSMAAPPSCSL

Approximately 85 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.