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HGFR Protein, Canine (HEK293, His)

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Based on 1 Customer Validation

The HGFR protein is a receptor tyrosine kinase that transduces signals by binding to hepatocyte growth factor/HGF. It regulates proliferation, scattering, morphogenesis, and cell survival. HGFR Protein, Canine (HEK293, His) is the recombinant canine-derived HGFR protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Canine
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HGFR Protein, Canine (HEK293, His)
  • Experimental Validation Results for HGFR Protein, Canine (HEK293, His)
  • HGFR Protein, Canine (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The HGFR protein is a receptor tyrosine kinase that transduces signals by binding to hepatocyte growth factor/HGF. It regulates proliferation, scattering, morphogenesis, and cell survival. HGFR Protein, Canine (HEK293, His) is the recombinant canine-derived HGFR protein, expressed by HEK293 , with N-His labeled tag.

Background

The HGFR protein, a receptor tyrosine kinase, serves as a critical transducer of signals from the extracellular matrix by binding to hepatocyte growth factor/HGF ligand. It plays a pivotal role in regulating various physiological processes, including proliferation, scattering, morphogenesis, and cell survival. Upon ligand binding at the cell surface, MET undergoes autophosphorylation on its intracellular domain, creating docking sites for downstream signaling molecules. Upon activation, HGFR interacts with PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3, or the adapter GAB1, leading to the activation of multiple signaling cascades, such as RAS-ERK, PI3 kinase-AKT, and PLCgamma-PKC. RAS-ERK activation is associated with morphogenetic effects, while PI3K/AKT coordinates prosurvival effects. In embryonic development, HGFR signaling contributes to gastrulation, muscle and neuronal precursor development and migration, angiogenesis, and kidney formation. In adults, it participates in wound healing, organ regeneration, tissue remodeling, and promotes the differentiation and proliferation of hematopoietic cells. Additionally, in the context of microbial infection, HGFR acts as a receptor for Listeria monocytogenes internalin InlB, facilitating the entry of the pathogen into cells.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Immobilized Canine HGFR at 5 μg/mL (100 μL/well) can bind Human HGF. The ED50 for this effect is 43.11 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Canine HGF R at 5 μg/mL (100 μL/well) can bind Human HGF. The ED50 for this effect is 43.11 ng/mL.

Technical Parameters

  • Species Canine
  • Source HEK293
  • Tag N-His
  • Accession

    Q75ZY9 (E25-L935)

  • Gene ID
    403438  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • HGFR (E25-L935)
      Accession # Q75ZY9
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    MET; Soluble MET Variant 13; MET Proto-Oncogene, Receptor Tyrosine Kinase; Soluble MET Variant 14; Hepatocyte Growth Factor Receptor; Soluble MET Variant 15; DFNB97; Soluble MET Variant 1; RCCP2; Soluble MET Variant 2; HGFR; Soluble MET Variant 3; Tyrosin

  • AA Sequence

    ECKEALVKSEMNVNMKYQLPNFTAETPIQNVVLHKHHIYLGAVNYIYVLNDKDLQKVAEYKTGPVLEHPDCSPCQDCSHKANLSGGVWEDNINMALLVDTYYDDQLISCGSVHRGTCQRHILPPSNIADIQSEVHCMYSSQADEEPSQCPDCVVSALGTKVLISEKDRFINFFVGNTINSSDHPDHSLHSISVRRLKETQDGFKFLTDQSYIDVLPEFRDSYPIKYVHAFESNHFIYFLTVQRETLDAQTFHTRIIRFCSVDSGLHSYMEMPLECILTEKRRKRSTREEVFNILQAAYVSKPGAHLAKQIGANLNDDILYGVFAQSKPDSAEPMNRSAVCAFPIKYVNEFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARNDEYRTEFTTALQRVDLFMGQFNQVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGLSTPHVNFRLDSHPVSPEAIVEHPLNQNGYTLVVTGKKITRIPLNGLGCEHFQSCSQCLSAPPFVQCGWCHDRCVHLEECPTGAWTQEVCLPAIYEVFPTSAPLEGGTVLTVCGWDFGFRRNNKFDLKKTKVFLGNESCTLTLSESTTNMLKCTVGPAVNEHFNISIIISNGRGTAQYSTFSYVDPIITSISPSYGPKNGGTLLTLTGKYLNSGNSRHISMGGKTCTLKSVSDSILECYTPAQATATEFPIKLKIDLANREMNSFSYQEDPIVYAIHPTKSFISGGSTITAVGKNLNSVSVLRMVIDVHETRRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGIHSKYFDLIYVHNPVFKPFEKPVMISIGNENVLEIKGNDIDPEAVKGEVLKVGNKSCETIYSDSKAVLCKVPNDLLKLNNELNIEWKQAVSSTVLGKVIVQPDQNFTGL

  • Molecular Weight

    Approximately 42-47 kDa & 85-95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Canine HGF R at 5 μg/mL (100 μL/well) can bind Human HGF. The ED50 for this effect is 43.11 ng/mL.

  • HGFR Protein, Canine (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for HGFR Protein, Canine (HEK293, His)
  • Experimental Validation Results for HGFR Protein, Canine (HEK293, His)
  • HGFR Protein, Canine (HEK293, His) Product Image