1. Recombinant Proteins
  2. Receptor Proteins
  3. Receptor Tyrosine Phosphatase
  4. IA-2/PTPRN
  5. IA2 Protein, Human (His)

IA2 Protein, Human (His)

Cat. No.: HY-P70848
COA Handling Instructions

The IA2 protein is critical in the vesicle-mediated secretion process and is essential for the accumulation of secretory vesicles in the hippocampus, pituitary gland, and pancreatic islets. IA2 is critical for insulin-related functions, ensuring normal levels of insulin-containing vesicles, preventing degradation and promoting glucose-stimulated insulin secretion. IA2 Protein, Human (His) is the recombinant human-derived IA2 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $67 In-stock
10 μg $115 In-stock
50 μg $320 In-stock
100 μg $545 In-stock
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Description

The IA2 protein is critical in the vesicle-mediated secretion process and is essential for the accumulation of secretory vesicles in the hippocampus, pituitary gland, and pancreatic islets. IA2 is critical for insulin-related functions, ensuring normal levels of insulin-containing vesicles, preventing degradation and promoting glucose-stimulated insulin secretion. IA2 Protein, Human (His) is the recombinant human-derived IA2 protein, expressed by E. coli , with N-6*His labeled tag.

Background

The IA2 protein plays a pivotal role in vesicle-mediated secretory processes, with essential functions extending across various tissues. It is required for the normal accumulation of secretory vesicles in key areas such as the hippocampus, pituitary, and pancreatic islets. Particularly crucial for insulin-related processes, IA2 is necessary for the accumulation of normal levels of insulin-containing vesicles, preventing their degradation and contributing to insulin secretion in response to glucose stimuli. IA2's influence also extends to neurotransmitter regulation in the brain, where it is required for the normal accumulation of norepinephrine, dopamine, and serotonin. Interestingly, in females, IA2 is uniquely indispensable for the normal accumulation and secretion of pituitary hormones like luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Moreover, IA2 plays a role in maintaining normal levels of renin expression and release. Despite lacking intrinsic enzyme activity, IA2 may regulate catalytic active protein-tyrosine phosphatases, such as PTPRA, through dimerization. The intricate dynamics of IA2 are further highlighted in its interaction with ICA512-TMF, where it modulates the dynamics and exocytosis of insulin secretory granules, proposing a tethering mechanism to the actin cytoskeleton dependent on UTRN and regulated by ICA512-CFF dimerization.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q16849-2 (R576-Q950)

Gene ID
Synonyms
Receptor-type tyrosine-protein phosphatase-like N; R-PTP-N; Islet cell antigen 512; ICA 512; Islet cell autoantigen 3; PTP IA-2; PTPRN; ICA3; ICA512
AA Sequence

RQQDKERLAALGPEGAHGDTTFEYQDLCRQHMATKSLFNRAEGPPEPSRVSSVSSQFSDAAQASPSSHSSTPSWCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

Molecular Weight

45-50 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

IA2 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IA2 Protein, Human (His)
Cat. No.:
HY-P70848
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