IL-2 Protein, Mouse

IL-2 protein is mainly produced by activated CD4-positive helper T cells and plays an important role in immune response and tolerance. Binding to IL-2R induces downstream signaling through JAK1 and JAK3 phosphorylation, thereby activating various pathways, including STAT, PI3K, and MAPK. IL-2 Protein, Mouse is the recombinant mouse-derived IL-2 protein, expressed by E. coli.

Interleukin-2 (IL-2) is a multi-faceted cytokine, known for promoting proliferation, survival, and cell death depending on the cell type and state. For example, IL-2 facilitates cell death only in activated T cells when antigen and IL-2 are abundant. The availability of IL-2 clearly impacts this process. IL-2 is retained in blood vessels by heparan sulfate, and that biologically active IL-2 is released from vessel tissue by heparanase. The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. IL-2 has a direct and unexpectedly broad influence on cellular homeostatic mechanisms in both immune and non-immune systems^[1].

IL-2 Protein, Mouse (1 μg/kg, ip, once daily for 5 days) increases the frequency and function of regulatory T cells (Tregs), improves the survival rate of mice in transplantation model^[2].
IL-2 Protein, Mouse (5 μg/kg, ip, once daily for 5 days) ameliorates the pulmonary edema and neutrophil aggregation, increases the level of anti-inflammatory cytokine IL-10 in mouse transfusion-related acute lung injury model^[3].

1.Measured in a cell proliferation assay using CTLL-2 Cells. The ED₅₀ for this effect is ≤0.5117 ng/mL, corresponding to a specific activity is ≥1.954×10⁶ units/mg.
2.Measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×10⁶ IU/mg.

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  Measured in a cell proliferation assay using CTLL-2 Cells. The ED₅₀ for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×10⁶ units/mg.

Mouse

E. coli

Tag Free

P04351 (A21-Q169)

16183  [NCBI]

Full Length of Mature Protein

APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

Approximately 17 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.02% Tween 80, pH 3.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.02% Tween 80, 4% mannitol, pH 3.0.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
4.Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.2% Tween 80, pH 3.0.
5.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
6.Lyophilized from a 0.22 μm filtered solution of 10 mM Sodium Citrate, pH 4.0.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/cho-cell.html  [Content Brief]

  [2]. Wrenshall LE, et al. Identification of a cytotoxic form of dimeric interleukin-2 in murine tissues. PLoS One. 2014 Jul 14;9(7):e102191.  [Content Brief]

  [3]. Tahvildari M, et al., Low-Dose IL-2 Therapy in Transplantation, Autoimmunity, and Inflammatory Diseases. J Immunol. 2019 Dec 1;203(11):2749-2755.  [Content Brief]

  [4]. He R, et al., Preventing murine transfusion-related acute lung injury by expansion of CD4+ CD25+ FoxP3+ Tregs using IL-2/anti-IL-2 complexes. Transfusion. 2019 Feb;59(2):534-544.  [Content Brief]