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  2. Enzymes & Regulators
  3. Protease Inhibitors Cystatin Family
  4. Kininogen-1
  5. Kininogen-1 Protein, Human (409a.a, HEK293, His)

Kininogen-1 Protein, Human (409a.a, HEK293, His)

Cat. No.: HY-P70956
COA Handling Instructions

Kininogen-1 is a key regulator in the coagulation process, inhibiting thiol proteases and strategically targeting prekallikrein and factor XI for coagulation regulation. HMW-kininogen prevents thrombin and plasmin-induced platelet aggregation and aids in hemostasis. Kininogen-1 Protein, Human (409a.a, HEK293, His) is the recombinant human-derived Kininogen-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kininogen-1 Protein, Human (409a.a, HEK293, His) is 409 a.a., with molecular weight of ~63.0 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Kininogen-1 is a key regulator in the coagulation process, inhibiting thiol proteases and strategically targeting prekallikrein and factor XI for coagulation regulation. HMW-kininogen prevents thrombin and plasmin-induced platelet aggregation and aids in hemostasis. Kininogen-1 Protein, Human (409a.a, HEK293, His) is the recombinant human-derived Kininogen-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kininogen-1 Protein, Human (409a.a, HEK293, His) is 409 a.a., with molecular weight of ~63.0 kDa.

Background

Kininogen-1, a pivotal player in blood coagulation, functions as an inhibitor of thiol proteases. In this capacity, HMW-kininogen strategically positions prekallikrein and factor XI adjacent to factor XII, crucial for blood clotting regulation. HMW-kininogen actively prevents thrombin- and plasmin-induced thrombocyte aggregation, contributing to hemostasis. In contrast, LMW-kininogen is not involved in blood clotting but exerts inhibition on thrombocyte aggregation. The active peptide bradykinin, released from HMW-kininogen, manifests diverse physiological effects, including influencing smooth muscle contraction, inducing hypotension, promoting natriuresis and diuresis, reducing blood glucose levels, mediating inflammation through increased vascular permeability, stimulating nociceptors, and triggering the release of other inflammatory mediators like prostaglandins. Additionally, bradykinin exhibits a cardioprotective effect, acting directly and indirectly through endothelium-derived relaxing factor action. The multifaceted roles of kininogen-1 underscore its significance in orchestrating various physiological processes.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P01042-2 (Q19-S427)

Gene ID
Molecular Construction
N-term
Kininogen-1 (Q19-S427)
Accession # P01042-2
6*His
C-term
Synonyms
Kininogen-1; lpha-2-Thiol Proteinase Inhibitor; Fitzgerald Factor; High Molecular Weight Kininogen; HMWK; Williams-Fitzgerald-Flaujeac Factor; KNG1; BDK; KNG
AA Sequence

QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTSHLRSCEYKGRPPKAGAEPASEREVS

Molecular Weight

Approximately 63.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM HAc-NaAC, 150 mM NaCl, pH 4.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Kininogen-1 Protein, Human (409a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Kininogen-1 Protein, Human (409a.a, HEK293, His)
Cat. No.:
HY-P70956
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