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LIFR Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

LIFR Protein, a signal-transducing molecule, may share a common pathway with IL6ST. Its soluble form acts as an inhibitor, blocking LIF binding to receptors and reducing biological activity. LIFR forms a heterodimer with IL6ST, interacting with the CNTF and CNTFR complex, indicating potential cross-talk and cooperative actions in cellular processes. LIFR Protein, Mouse (HEK293, His) is the recombinant mouse-derived LIFR protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for LIFR Protein, Mouse (HEK293, His)
  • Experimental Validation Results for LIFR Protein, Mouse (HEK293, His)
  • LIFR Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

LIFR Protein, a signal-transducing molecule, may share a common pathway with IL6ST. Its soluble form acts as an inhibitor, blocking LIF binding to receptors and reducing biological activity. LIFR forms a heterodimer with IL6ST, interacting with the CNTF and CNTFR complex, indicating potential cross-talk and cooperative actions in cellular processes. LIFR Protein, Mouse (HEK293, His) is the recombinant mouse-derived LIFR protein, expressed by HEK293 , with C-His labeled tag.

Background

LIFR protein is a signal-transducing molecule that potentially shares a common pathway with IL6ST. Its soluble form acts as an inhibitor, blocking the binding of LIF to receptors on target cells, thus dampening its biological activity. LIFR forms a heterodimer with IL6ST, and this complex interacts with the complex formed by CNTF and CNTFR, suggesting potential cross-talk and cooperative actions in cellular processes.

Verified Bioactivity

Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.051 μg/mL, corresponding to a specific activity is 9.51×10^2 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.051 μg/mL, corresponding to a specific activity is 9.51×10^2 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P42703/NP_038612.1 (L44-S828)

  • Gene ID
    16880  [NCBI]
  • Molecular Construction
    • N-term
    • LIFR (L44-S828)
      Accession # P42703/NP_038612.1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    LIFR; CD118 Antigen; LIF Receptor Subunit Alpha; LIF-R; Leukemia Inhibitory Factor Receptor; LIF Receptor; CD118; SJS2; Leukemia Inhibitory Factor Receptor Alpha; STWS; LIF Receptor Alpha; SWS

  • AA Sequence

    LKRGVQDLKCTTNNMRVWDCTWPAPLGVSPGTVKDICIKDRFHSCHPLETTNVKIPALSPGDHEVTINYLNGFQSKFTLNEKDVSLIPETPEILDLSADFFTSSLLLKWNDRGSALPHPSNATWEIKVLQNPRTEPVALVLLNTMLSGKDTVQHWNWTSDLPLQCATHSVSIRWHIDSPHFSGYKEWSDWSPLKNISWIRNTETNVFPQDKVVLAGSNMTICCMSPTKVLSGQIGNTLRPLIHLYGQTVAIHILNIPVSENSGTNIIFITDDDVYGTVVFAGYPPDVPQKLSCETHDLKEIICSWNPGRITGLVGPRNTEYTLFESISGKSAVFHRIEGLTNETYRLGVQMHPGQEIHNFTLTGRNPLGQAQSAVVINVTERVAPHDPTSLKVKDINSTVVTFSWYLPGNFTKINLLCQIEICKANSKKEVRNATIRGAEDSTYHVAVDKLNPYTAYTFRVRCSSKTFWKWSRWSDEKRHLTTEATPSKGPDTWREWSSDGKNLIVYWKPLPINEANGKILSYNVSCSLNEETQSVLEIFDPQHRAEIQLSKNDYIISVVARNSAGSSPPSKIASMEIPNDDITVEQAVGLGNRIFLTWRHDPNMTCDYVIKWCNSSRSEPCLLDWRKVPSNSTETVIESDQFQPGVRYNFYLYGCTNQGYQLLRSIIGYVEELAPIVAPNFTVEDTSADSILVKWDDIPVEELRGFLRGYLFYFQKGERDTPKTRSLEPHHSDIKLKNITDISQKTLRIADLQGKTSYHLVLRAYTHGGLGPEKSMFVVTKENS

  • Molecular Weight

    Approximately 110-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.051 μg/mL, corresponding to a specific activity is 9.51×10^2 units/mg.

  • LIFR Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for LIFR Protein, Mouse (HEK293, His)
  • Experimental Validation Results for LIFR Protein, Mouse (HEK293, His)
  • LIFR Protein, Mouse (HEK293, His) Product Image