PD-L2 Protein, Human (HEK293, His)

PD-L2 Protein, operating independently of PDCD1, plays a vital role in T-cell proliferation and IFNG production. Its interaction with PDCD1 inhibits proliferation and cytokine production. The interplay between PD-L2 and PDCD1 regulates immune responses, influencing T-cell activation and function. This molecular interaction adds complexity to mechanisms governing T-cell behavior, highlighting PD-L2's versatile role in immune regulation. PD-L2 Protein, Human (HEK293, His) is the recombinant human-derived PD-L2 protein, expressed by HEK293 , with C-His labeled tag.

PD-L2 Protein is integral to the costimulatory signal crucial for T-cell proliferation and IFNG production, operating in a PDCD1-independent manner. Its interaction with PDCD1, however, functions to inhibit T-cell proliferation by impeding cell cycle progression and cytokine production. The intricate interplay between PD-L2 and PDCD1 underscores its role as a regulatory checkpoint in modulating immune responses, influencing the activation and function of T cells. This molecular interaction adds a layer of complexity to the dynamic mechanisms governing T-cell behavior, highlighting PD-L2's versatile role in immune regulation.

1.Measured by its binding ability in a functional ELISA. Immobilized human PD-L2 at 2 μg/mL(100 μl/well) can bind human PD-1, the EC₅₀ of human PD-1 is 150-800 ng/mL.
2.Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L2 is coated at 1 μg/mL (100 μL/well) can bind Recombinant Human PD-1 (HY-P73344). The ED₅₀ for this effect is ≤0.5 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L2 is coated at 1 μg/mL (100 μL/well) can bind Recombinant Human PD-1 (HY-P73344). The ED₅₀ for this effect is 0.369 μg/mL.

Human

HEK293

C-His

Q9BQ51-1 (L20-P219)

80380  [NCBI]

Extracellular Domain

LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHP

Approximately 33-52 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.