Protein Kinase D2/PRKD2 Protein, Human (Active, sf9, His-GST)

Protein kinase D2 (PRKD2) is a serine/threonine protein kinase that mediates various cellular processes downstream of PKC. It affects cell proliferation through MAPK1/3 (ERK1/2) signaling, activates NF-kappa-B under oxidative stress, inhibits HDAC7 transcriptional repression, and regulates T cell antigen receptor (TCR) signaling, cytokine production, Golgi trafficking, angiogenesis, particle release, and cell adhesion. Protein Kinase D2/PRKD2 Protein, Human (sf9, His-GST) is the recombinant human-derived protein Kinase D2/PRKD2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Protein Kinase D2 (PRKD2), a serine/threonine-protein kinase, acts as a pivotal mediator of diverse cellular processes. It translates transient diacylglycerol (DAG) signals into sustained physiological effects downstream of PKC, influencing cell proliferation through MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, T-cell antigen receptor (TCR) and cytokine production, Golgi membrane trafficking, angiogenesis, secretory granule release, and cell adhesion. PRKD2 may enhance mitogenesis induced by neuropeptide bombesin by prolonging MAPK1/3 signaling, leading to the accumulation of immediate-early gene products, including FOS, promoting cell cycle progression. Phosphorylation of PRKD2 at Tyr-438 and Tyr-717 by ABL1 in response to oxidative stress activates NF-kappa-B. Additionally, PRKD2, in response to gastrin receptor CCKBR activation, phosphorylates HDAC7, inhibiting its transcriptional repression of NR4A1/NUR77. The kinase also plays essential roles in T-cell activation, angiogenesis, secretion, and tumor growth, demonstrating its multifaceted involvement in cellular functions.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. When the protein concentration is 0.63 nM, the conversion rate is about 20%.

Human

Sf9 insect cells

N-His;N-GST

Q9BZL6-1/ NP_057541.2 (M1-L878)

25865  [NCBI]

Full Length of Isoform-1

MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL

Approximately 120 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, 20% glycerol, 5 mM DTT, 0.1 M Trehalose.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.