Copy Link Print Information

Serpin E1 Protein, Mouse (HEK293, His)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Serpin E1 protein is a serine protease inhibitor that regulates TMPRSS7 and is a major inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU).It can significantly downregulate fibrinolysis and control thrombus degradation.Serpin E1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin E1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Serpin E1 Protein, Mouse (HEK293, His)
  • Serpin E1 Protein, Mouse (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Serpin E1 protein is a serine protease inhibitor that regulates TMPRSS7 and is a major inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU).It can significantly downregulate fibrinolysis and control thrombus degradation.Serpin E1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin E1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Serpin E1, a serine protease inhibitor, exerts its regulatory functions through various mechanisms. It inhibits TMPRSS7 and serves as a primary inhibitor for both tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). Acting as a crucial PLAT inhibitor, it plays a vital role in down-regulating fibrinolysis and orchestrates the controlled degradation of blood clots. In its capacity as a PLAU inhibitor, Serpin E1 is implicated in the regulation of cell adhesion and spreading. Beyond its role as a protease inhibitor, it acts as a regulator of cell migration, notably contributing to the stimulation of keratinocyte migration during cutaneous injury repair. Furthermore, Serpin E1 is involved in cellular and replicative senescence, playing a role in alveolar type 2 cells' senescence in the lung. Additionally, it participates in the regulation of cementogenic differentiation of periodontal ligament stem cells and influences odontoblast differentiation and dentin formation during odontogenesis. Serpin E1 forms a heterodimer with TMPRSS7, interacts with various proteins like VTN, PPP1CB, SORL1, LRP1, and PLAUR, and engages in complex interactions in different cellular contexts, emphasizing its multifaceted regulatory role.

Verified Bioactivity

Measured by its ability to inhibit uPA (HY-P78017) cleavage of a peptide substrate,N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin(Z-GGR-AMC) (HY-P0019A) at room temperature for 15 minutes. The IC50 value is <13 nM.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Assay Procedure

Materials
Assay buffer: 50 mM Tris, 0.01% (v/v) Tween? 20, pH 8.5
Serpin E1 Protein, Mouse (HEK293, His) (HY-P71133)
PLAU/uPA Protein, Human (411a.a, HEK293, His, solution) (HY-P71050)
Substrate: Z-Gly-Gly-Arg-AMC acetate (HY-P0019A)

Procedure
1. Dilute Human uPA to 2 μg/mL with assay buffer.
2. Prepare different concentrations of Human Serpin E1 with assay buffer for the following serial dilutions: 1500, 750, 375, 187.5, 125, 75, 50, 25, and 12.5 nM.
3. Equal volumes of Human Serpin E1/PAI-1 curves were mixed with diluted Human uPA at 2 μg/mL. Two controls containing only Human uPA and assay buffer were included.
4. The above mixture was incubated for 15 minutes at room temperature.
5. After incubation, the mixture was diluted 10-fold in assay buffer.
6. Dilute the substrate to 200 μM with assay buffer.
7. In a 96-well plate, add 50 μL of reaction mixture and start the reaction with 50 μL of 20 μM substrate.
8. Excitation and emission wavelengths 380 nm and 460 nm , read in kinetic mode for 5 minutes.
9. The 50% inhibitory concentration (IC50) of Human Serpin E1 was derived by plotting RFU/min (or specific activity) versus concentration using a 4-PL fit.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    G5E899 (T23-P402)

  • Gene ID
    18787  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin E1 (T23-P402)
      Accession # G5E899
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SERPINE1; Endothelial Plasminogen Activator Inhibitor; Prev. PLANH1; Serpin E1; Prev. PAI1; PAI-1; PAI; Serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1; Plasminogen Activator Inhibitor, Type I; Serpin Family E Member 1; Plasminogen Activator Inhibitor 1

  • AA Sequence

    TLPLRESHTAHQATDFGVKVFQQVVQASKDRNVVFSPYGVSSVLAMLQMTTAGKTRRQIQDAMGFKVNEKGTAHALRQLSKELMGPWNKNEISTADAIFVQRDLELVQGFMPHFFKLFQTMVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVDELTRLVLVNALYFSGQWKTPFLEASTHQRLFHKSDGSTVSVPMMAQSNKFNYTEFTTPDGLEYDVVELPYQGDTLSMFIAAPFEKDVHLSALTNILDAELIRQWKGNMTRLPRLLILPKFSLETEVDLRGPLEKLGMPDMFSATLADFTSLSDQEQLSVAQALQKVRIEVNESGTVASSSTAFVISARMAPTEMVIDRSFLFVVRHNPTETILFMGQVMEP

  • Predicted Molecular Mass

    43.7 kDa

  • Molecular Weight

    Approximately 45-54 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Serpin E1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Serpin E1 Protein, Mouse (HEK293, His)
  • Serpin E1 Protein, Mouse (HEK293, His) Product Image