Serpin A1b Protein, Mouse (HEK293, Fc)

Serpin A1b Protein, a vigilant guardian in cellular regulation, adeptly inhibits elastase, providing formidable defense against its enzymatic activity. Beyond this specialization, Serpin A1b shows moderate affinity for plasmin and thrombin, highlighting its versatility in interacting with various serine proteases. As a multifaceted sentinel, Serpin A1b contributes to the nuanced orchestration of proteolytic processes within cellular landscapes. Serpin A1b Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Serpin A1b protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Serpin A1b Protein, Mouse (HEK293, Fc) is 389 a.a., with molecular weight of ~70.2 kDa.

Serpin A1b, a vigilant guardian within the intricate realm of cellular regulation, operates as an adept inhibitor of serine proteases. Elastase stands as its primary target, encountering a formidable defense against its enzymatic activity. Beyond its specialized role, Serpin A1b also demonstrates a moderate affinity for plasmin and thrombin, showcasing its versatility in interacting with a spectrum of serine proteases. In fulfilling its regulatory duties, Serpin A1b emerges as a multifaceted sentinel, contributing to the nuanced orchestration of proteolytic processes within cellular landscapes.

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC₅₀ value is 4.166 nM, as measured under the described conditions.

Mouse

HEK293

C-hFc

P22599 (E25-K413)

20701  [NCBI]

EDVQETDTSQKDQSPASHEIATNLGDFAISLYRELVHQSNTSNIFFSPVSIATAFAMLSLGSKGDTHTQILEGLQFNLTQTSEADIHKSFQHLLQTLNRPDSELQLSTGNGLFVNNDLKLVEKFLEEAKNHYQAEVFSVNFAESEEAKKVINDFVEKGTQGKIVEAVKELDQDTVFALANYILFKGKWKKPFDPENTEEAEFHVDKSTTVKVPMMMLSGMLDVHHCSILSSWVLLMDYAGNASAVFLLPEDGKMQHLEQTLNKELISKILLNRRRRLVQIHIPRLSISGDYNLKTLMSPLGITRIFNNGADLSGITEENAPLKLSKAVHKAVLTIDETGTEAAAATVFEAVPMSMPPILRFDHPFLFIIFEEHTQSPIFVGKVVDPTHK

Approximately 70-100 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.