1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Angiotensinogen
  6. Serpin A8/Angiotensinogen Protein, Human (HEK293, His)

Serpin A8/Angiotensinogen Protein, Human (HEK293, His)

Cat. No.: HY-P73653
COA Handling Instructions

Serpin A8/angiotensinogen protein is an important component of the renin-angiotensin system (RAS), which regulates blood pressure, fluid balance, and electrolyte homeostasis. Serpin A8/Angiotensinogen Protein, Human (HEK293, His) is the recombinant human-derived Serpin A8/Angiotensinogen protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin A8/Angiotensinogen Protein, Human (HEK293, His) is 452 a.a., with molecular weight of 52-64 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Serpin A8/angiotensinogen protein is an important component of the renin-angiotensin system (RAS), which regulates blood pressure, fluid balance, and electrolyte homeostasis. Serpin A8/Angiotensinogen Protein, Human (HEK293, His) is the recombinant human-derived Serpin A8/Angiotensinogen protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin A8/Angiotensinogen Protein, Human (HEK293, His) is 452 a.a., with molecular weight of 52-64 kDa.

Background

Serpin A8, also known as angiotensinogen, stands as an indispensable element within the renin-angiotensin system (RAS), a potent regulatory framework governing blood pressure, body fluid, and electrolyte homeostasis. Operating directly on vascular smooth muscle, it exerts a formidable vasoconstrictive influence and dynamically influences cardiac contractility and heart rate through interactions with the sympathetic nervous system. Additionally, angiotensinogen plays a pivotal role in shaping renal sodium and water absorption, eliciting its effects by stimulating zona glomerulosa cells in the adrenal cortex to synthesize and release aldosterone. This multifaceted functionality is mediated through the binding of angiotensinogen to angiotensin receptors AGTR1 and AGTR2, orchestrating a cascade of physiological responses. Beyond its pivotal role in the RAS, angiotensinogen exhibits an ability to engage with the DEAR/FBXW7-AS1 receptor, expanding its repertoire of molecular interactions and suggesting broader implications for its regulatory influence.

Biological Activity

Measured in a cell proliferation assay using HT-29 human coloncancer cells. The ED50 for this effect is 160.2 ng/mL, corresponding to a specificactivity is 6242.19 Unit/mg.

  • Measured in a cell proliferation assay using HT-29 human coloncancer cells.The ED50 for this effect is 160.2 ng/mL, corresponding to a specificactivity is 6242.19 unit/mg.
Species

Human

Source

HEK293

Tag

C-His

Accession

P01019 (D34-A485)

Gene ID

183  [NCBI]

Molecular Construction
N-term
ANGT (D34-A485)
Accession # P01019
His
C-term
Synonyms
Angiotensinogen; Serpin A8; AGT; SERPINA8
AA Sequence

DRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLMGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVPFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA

Molecular Weight

52-64 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Serpin A8/Angiotensinogen Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Serpin A8/Angiotensinogen Protein, Human (HEK293, His)
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HY-P73653
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