Serpin A8/Angiotensinogen Protein, Human (HEK293, His)

Serpin A8/angiotensinogen protein is an important component of the renin-angiotensin system (RAS), which regulates blood pressure, fluid balance, and electrolyte homeostasis. Serpin A8/Angiotensinogen Protein, Human (HEK293, His) is the recombinant human-derived Serpin A8/Angiotensinogen protein, expressed by HEK293 , with C-His labeled tag.

Serpin A8, also known as angiotensinogen, stands as an indispensable element within the renin-angiotensin system (RAS), a potent regulatory framework governing blood pressure, body fluid, and electrolyte homeostasis. Operating directly on vascular smooth muscle, it exerts a formidable vasoconstrictive influence and dynamically influences cardiac contractility and heart rate through interactions with the sympathetic nervous system. Additionally, angiotensinogen plays a pivotal role in shaping renal sodium and water absorption, eliciting its effects by stimulating zona glomerulosa cells in the adrenal cortex to synthesize and release aldosterone. This multifaceted functionality is mediated through the binding of angiotensinogen to angiotensin receptors AGTR1 and AGTR2, orchestrating a cascade of physiological responses. Beyond its pivotal role in the RAS, angiotensinogen exhibits an ability to engage with the DEAR/FBXW7-AS1 receptor, expanding its repertoire of molecular interactions and suggesting broader implications for its regulatory influence.

Measured in a cell proliferation assay using HT-29 human coloncancer cells. The ED₅₀ for this effect is 160.2 ng/mL, corresponding to a specificactivity is 6242.19 Unit/mg.

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  Measured in a cell proliferation assay using HT-29 human coloncancer cells.The ED₅₀ for this effect is 160.2 ng/mL, corresponding to a specificactivity is 6242.19 unit/mg.

Human

HEK293

C-His

NP_000020.1 (D34-A485)

183  [NCBI]

Partial

DRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLMGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVPFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA

52-64 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.