Serpin A8/Angiotensinogen Protein, Rat (HEK293, His)

Serpin A8 (or angiotensinogen) is a key regulator of the renin-angiotensin system, affecting blood pressure, fluid and electrolyte balance. As a vasoconstrictor, it directly affects vascular smooth muscle and affects myocardial contractility and heart rate through the sympathetic nervous system. Serpin A8/Angiotensinogen Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin A8/Angiotensinogen protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin A8/Angiotensinogen Protein, Rat (HEK293, His) is 453 a.a., with molecular weight of 58-75 kDa.

Serpin A8, also known as angiotensinogen, stands as an essential component within the intricate network of the renin-angiotensin system (RAS), serving as a potent regulator of blood pressure, body fluid, and electrolyte homeostasis. This multifaceted protein exerts its influence on various physiological processes. Directly impacting vascular smooth muscle, it functions as a formidable vasoconstrictor, affecting cardiac contractility and heart rate through interactions with the sympathetic nervous system. Furthermore, angiotensinogen plays a crucial role in renal function by modulating sodium and water absorption, achieved by stimulating adrenal cortex zona glomerulosa cells to synthesize and release aldosterone. These diverse actions are mediated by its binding to angiotensin receptors AGTR1 and AGTR2. Beyond its role in the RAS, angiotensinogen demonstrates additional interactions, such as binding to the DEAR/FBXW7-AS1 receptor, expanding its involvement in cellular signaling networks.

Rat

HEK293

C-10*His

P01015 (D25-V477)

24179  [NCBI]

DRVYIHPFHLLYYSKSTCAQLENPSVETLPEPTFEPVPIQAKTSPVDEKTLRDKLVLATEKLEAEDRQRAAQVAMIANFMGFRMYKMLSEARGVASGAVLSPPALFGTLVSFYLGSLDPTASQLQVLLGVPVKEGDCTSRLDGHKVLTALQAVQGLLVTQGGSSSQTPLLQSTVVGLFTAPGLRLKQPFVESLGPFTPAIFPRSLDLSTDPVLAAQKINRFVQAVTGWKMNLPLEGVSTDSTLFFNTYVHFQGKMRGFSQLTGLHEFWVDNSTSVSVPMLSGTGNFQHWSDAQNNFSVTRVPLGESVTLLLIQPQCASDLDRVEVLVFQHDFLTWIKNPPPRAIRLTLPQLEIRGSYNLQDLLAQAKLSTLLGAEANLGKMGDTNPRVGEVLNSILLELQAGEEEQPTESAQQPGSPEVLDVTLSSPFLFAIYERDSGALHFLGRVDNPQNVV

Approximately 58-75 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.