Siglec-2/CD22 Protein, Mouse (HEK293, Fc)

Siglec-2/CD22 Protein, Mouse (690a.a, HEK293, Fc) is a Siglec of ~140 kDa with seven Ig-like domains and is expressed in a B-cell lineage-specific fashion, starting at the pre-B cell stage. CD22 is associated with membrane-bound IgM on the surface of B cells. Siglec-2 (CD22) specifically recognizes 伪2.6 sialic acid-containing glycans. Siglec-2 negatively regulates B cell antigen receptor (BCR) signaling by recruitment and activation of SH2-containing phosphatase 1 (SHP-1), which counteracts the phospharylation-mediated activation of signaling molecules by dephosphorylation, and reduces Ca²⁺ signaling by regulating the activity of the Ca²⁺ pump PMCA^[1][2]. Siglec-2/CD22 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with C-hFc labeled tag.

Siglec-2 (CD22) specifically recognizes α2.6 sialic acid-containing glycans and is expressed on B cells, the precursors of antibody secreting cells, plasma cells. Siglec-2 negatively regulates signaling through the B cell antigen receptor (BCR), which plays a crucial role in response of B cells to antigen stimulation. Siglec-2 negatively regulates BCR signaling by recruitment and activation of SH2-containing phosphatase 1 (SHP-1), which counteracts the phospharylation-mediated activation of signaling molecules by dephosphorylation, and reduces Ca2+ signaling by regulating the activity of the Ca2+ pump PMCA. After stimulation of the BCR by antigen, CD22 is tyrosine-phosphorylated on its intracellular tail. CD22 exhibits hallmarks of clathrin-mediated endocytosis and traffics to recycling compartments^[1][2].

Mouse

HEK293

C-hFc

BAE33829 (R19-R708)

12483  [NCBI]

Partial

RYSSANDWTVDHPQTLFAWEGACIRIPCKYKTPLPKARLDNILLFQNYEFDKATKKFTGTVLYNATKTEKDPESELYLSKQGRVTFLGNRIDNCTLKIHPIRANDSGNLGLRMTAGTERWMEPIHLNVSEKPFQPYIQMPSEIRESQSVTLTCGLNFSCFGYDILLKWFLEDSEITSITSSVTSITSSVTSSIKNVYTESKLTFQPKWTDHGKSVKCQVQHSSKVLSECTVHLDVKYTPKLEIKVNPTEVEKNNSVTMTCRVNSSNPKLRTVAVSWFKDGRPLEDQELEQEQQMSKLILHSVTKDMRGKYRCQASNDIGPGESEEVELTVHYAPEPSRVHIYPSPAEEGQSVELICESLASPSATNYTWYHNRKPIPGDTQEKLRIPKVSPWHAGNYSCLAENRLGHGKIDQEAKLDVHYAPKAVTTVIQSFTPILEGDSVTLVCRYNSSNPDVTSYRWNPQGSGSVLKPGVLRIQKVTWDSMPVSCAACNHKCSWALPVILNVHYAPRDVKVLKVSPASEIRAGQRVLLQCDFAESNPAEVRFFWKKNGSLVQEGRYLSFGSVSPEDSGNYNCMVNNSIGETLSQAWNLQVLYAPRRLRVSISPGDHVMEGKKATLSCESDANPPISQYTWFDSSGQDLHSSGQKLRLEPLEVQHTGSYRCKGTNGIGTGESPPSTLTVYYSPETIGKR

Approximately 125 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.