2. Recombinant Proteins
  3. CD Antigens Receptor Proteins Biotinylated Proteins
  4. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  5. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  6. SIRP alpha V2/CD172a Protein, Human (Biotinylated, HEK293, His-Avi)

SIRP alpha V2/CD172a Protein, Human (Biotinylated, HEK293, His-Avi)

Art. -Nr.: HY-P77840
Handling Instructions Technical Support

SIRP alpha V2/CD172a Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived SIRP alpha V2/CD172a protein, expressed by HEK293, with C-Avi, C-His labeled tag.

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Beschreibung

SIRP alpha V2/CD172a Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived SIRP alpha V2/CD172a protein, expressed by HEK293, with C-Avi, C-His labeled tag.
Species

Human
Source

HEK293
Tag

C-Avi;C-6*His
Accession

AAH26692.1 (E31-R369)
Gene ID
Molecular Construction
N-term
CD172a (E31-R369)
Accession # AAH26692.1
6*His-Avi
C-term
Protein Length

Partial
Conjugation

Biotin
Synonyms
CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
AA Sequence

EEELQVIQPDKSVSVAAGESAILHCTVTSLIPVGPIQWFRGAGPARELIYNQKEGHFPRVTTVSESTKRENMDFSISISNITPADAGTYYCVKFRKGSPDTEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

Predicted Molecular Mass
40 kDa
Molekulargewicht

Approximately 55-65 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Reinheit

≥ 95%, as determined by Bis-Tris PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Dokumentation
Handling Instructions (2659 KB)

SIRP alpha V2/CD172a Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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