1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  3. Angiopoietins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TIE-2/CD202b TIE Receptors
  5. TIE-2
  6. TIE-2 Protein, Mouse (HEK293, Fc)

TIE-2 Protein, Mouse (HEK293, Fc)

Cat. No.: HY-P73437
Handling Instructions

TIE-2 is a tyrosine protein kinase that serves as a cell surface receptor for ANGPT1, ANGPT2, and ANGPT4 and is critical for angiogenesis, endothelial cell processes, and vascular stability. In addition to embryonic vasculogenesis, TIE-2 also affects postnatal hematopoiesis, regulating angiogenesis depending on the situation. TIE-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TIE-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIE-2 Protein, Mouse (HEK293, Fc) is 744 a.a., with molecular weight of ~107.7 kDa.

For research use only. We do not sell to patients.

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Description

TIE-2 is a tyrosine protein kinase that serves as a cell surface receptor for ANGPT1, ANGPT2, and ANGPT4 and is critical for angiogenesis, endothelial cell processes, and vascular stability. In addition to embryonic vasculogenesis, TIE-2 also affects postnatal hematopoiesis, regulating angiogenesis depending on the situation. TIE-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TIE-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIE-2 Protein, Mouse (HEK293, Fc) is 744 a.a., with molecular weight of ~107.7 kDa.

Background

TIE-2 protein, a tyrosine-protein kinase, acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4, orchestrating a comprehensive range of processes critical for angiogenesis, endothelial cell survival, proliferation, migration, adhesion, and cell spreading, as well as the maintenance of vascular quiescence. Beyond its essential role in embryonic angiogenesis and heart development, TIE-2 plays a vital role in post-natal hematopoiesis. Its function post-birth involves context-dependent activation or inhibition of angiogenesis. In quiescent vessels, ANGPT1 oligomers recruit TIE-2 to cell-cell contacts, fostering complex formation with neighboring TIE-2 molecules and preferential activation of the phosphatidylinositol 3-kinase and AKT1 signaling cascades, leading to vascular stability. Conversely, in migrating endothelial cells lacking cell-cell adhesions, ANGPT1 recruits TIE-2 to contacts with the extracellular matrix, activating focal adhesion complexes, PTK2/FAK, and downstream kinases MAPK1/ERK2 and MAPK3/ERK1, stimulating sprouting angiogenesis. ANGPT1-triggered TIE-2 signaling involves receptor dimerization and autophosphorylation at specific tyrosine residues, serving as binding sites for scaffold proteins and effectors. Modulation by ANGPT2, which competes for the same binding site, and formation of heterodimers with TIE1, as well as proteolytic processing yielding a soluble extracellular domain, further regulate TIE-2 signaling. The soluble extracellular domain functions as a decoy receptor for angiopoietins, influencing signaling dynamics. TIE-2 phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1, and TIE1, underscoring its intricate role in finely tuning a myriad of cellular responses.

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

Q02858 (M1-K744)

Gene ID

21687  [NCBI]

Molecular Construction
N-term
TIE-2 (M1-K744)
Accession # Q02858
hFc
C-term
Synonyms
Angiopoietin-1 receptor; CD202b; hTIE2; p140 TEK; Tie2; VMCM
Molecular Weight

Approximately 107.7 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TIE-2 Protein, Mouse (HEK293, Fc)
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HY-P73437
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